Anti-Dopamine β Hydroxylase Antibody, clone 2B11 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1328 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human adrenal gland (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1328, Anti-Dopamine β Hydroxylase, clone 2B11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/ECM staining was observed in medullary cells of human adrenal gland tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human adrenal gland (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1328, Anti-Dopamine β Hydroxylase, clone 2B11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/ECM staining was observed in medullary cells of human adrenal gland tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human adrenal gland (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1328, Anti-Dopamine β Hydroxylase, clone 2B11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/ECM staining was observed in medullary cells of human adrenal gland tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U2OS cells was performed using a 1:100 dilution of Cat. No. ZRB1328, Anti-Dopamine β Hydroxylase, clone 2B11 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the endoplasmic reticulum & vesicles.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U2OS cells was performed using a 1:100 dilution of Cat. No. ZRB1328, Anti-Dopamine β Hydroxylase, clone 2B11 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the endoplasmic reticulum & vesicles.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U2OS cells was performed using a 1:100 dilution of Cat. No. ZRB1328, Anti-Dopamine β Hydroxylase, clone 2B11 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the endoplasmic reticulum & vesicles.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from SH-SY5Y cells (Lane 1), Mouse Adrenal gland (Lane 2), Rat Adrenal gland (Lane 3), and Human Adrenal gland (Lane 4) were probed with Cat. No. ZRB1328, Anti-Dopamine β Hydroxylase, clone 2B11 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Dopamine β Hydroxylase (~75 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Dopamine β Hydroxylase peptide (Run 2) Cat. No. ZRB1328, Anti-Dopamine β Hydroxylase, clone 2B11 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Dopamine β Hydroxylase peptide displayed a KD of 1.0 x 10-12 whereas binding to non-specific control peptide displayed a KD of 9.2 x 10-7.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2B11, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminus |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 69.07 kDa |
| observed mol wt ~75 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_000778  |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, human, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P09172 |
| Application: | Quality Control Testing Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Dopamine β Hydroxylase in SH-SY5Y cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Dopamine β Hydroxylase in mouse, rat, and human adrenal gland tissue lysates. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Dopamine β Hydroxylase in human adrenal gland tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Dopamine β Hydroxylase in U2OS cells. Affinity Binding Assay: A representative lot of this antibody bound Dopamine β Hydroxylase peptide with a KD of 1.0 x 10-12 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | This rabbit monoclonal Anti-Dopamine β Hydroxylase, clone 2B11 ZooMAb® Rabbit Monoclonal, Cat. No. ZRB1328 is used in Western Blotting, Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, for detection of Dopamine β Hydroxylase. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Dopamine β Hydroxylase. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2B11 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Dopamine β Hydroxylase. It targets an epitope within 18 amino acids from the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Dopamine β-hydroxylase (UniProt: P09172; also known as EC:1.14.17.1, Dopamine beta-monooxygenase) is encoded by the DBH gene (Gene ID: 1621) in human. Dopamine β-hydroxylase is a copper-containing homotetrametic oxygenase that catalyzes the hydroxylation of the β-carbon of a wide variety of phenylethylamine derivatives, using oxygen and ascorbic acid as cofactors. It is responsible for conversion of dopamine to norepinephrine (NE). It is generally localized in the chromaffin granules of the adrenal medulla and sympathetic neurons of the central and peripheral nervous system. Proteolytic cleave at the G-S site (amino acids 39-40) leads to the release of a soluble form of enzyme. Dopamine β-hydroxylase activity is low at birth and reaches increases significantly by four weeks of age. Subjects with Dopamine β-hydroxylase deficiency display low levels of NE and its metabolites in plasma, cerebrospinal fluid, and urine. Due to the fact that NE normally acts as an inhibitor of tyrosine hydroxylase, patients with Dopamine β-hydroxylase deficiency show higher levels of dopamine. Mutations in DBH gene are known to cause orthostatic hypotension (postural hypotension) characterized by reduced systolic and diastolic pressure upon rising from supine to standing position. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |