Anti-BLBP/FABP7 Antibody, clone 7I23, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB13190 - recombinant monoclonal antibody
Synonym: B-FABP; BLBP; Brain lipid-binding protein; Brain-type fatty acid-binding protein; Fatty acid-binding protein; Fatty acid-binding protein 7; Mammary-derived growth inhibitor related
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human brain (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB13190, Anti-BLBP/FABP7, clone 7I23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous/nuclear staining was observed in glial cells of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human brain (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB13190, Anti-BLBP/FABP7, clone 7I23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous/nuclear staining was observed in glial cells of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human brain (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB13190, Anti-BLBP/FABP7, clone 7I23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous/nuclear staining was observed in glial cells of human cerebral cortex tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human cerebellum tissue sections was performed using a 1:100 dilution of Cat. No. ZRB13190, Anti-BLBP/FABP7, clone 7I23 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). Cytoplasmic/membranous/nuclear staining was observed in granular and molecular layers of human cerebellum tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human cerebellum tissue sections was performed using a 1:100 dilution of Cat. No. ZRB13190, Anti-BLBP/FABP7, clone 7I23 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). Cytoplasmic/membranous/nuclear staining was observed in granular and molecular layers of human cerebellum tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human cerebellum tissue sections was performed using a 1:100 dilution of Cat. No. ZRB13190, Anti-BLBP/FABP7, clone 7I23 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). Cytoplasmic/membranous/nuclear staining was observed in granular and molecular layers of human cerebellum tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Rat (Lane 1), Mouse (Lane 2), and human (Lane 3) brain tissue lysates were probed with Cat. No. ZRB13190, Anti-BLBP/FABP7, clone 7I23 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates BLBP/FABP7 (~15 kDa).
Western Blotting Enhanced Validation - Recombinant Antibody Technology FABP2 Recombinant (Lane 1), FABP3 Recombinant (Lane 2), FABP 4 Recombinantand (Lane 3), and GST/His-tagged FABP7 Recombinant (Lane 4) proteins were probed with Cat. No. ZRB13190, Anti-BLBP/FABP7, clone 7I23 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates GST/His-tagged BLBP/FABP7 (~40 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 7I23, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 14.89 kDa |
| observed mol wt ~15 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| shipped in | ambient |
| species reactivity | rat, human, mouse |
| storage temp. | 2-8°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | O15540  |
| Application: | Anti-BLBP/FABP7, clone 7I23 ZooMAb, Cat. No. ZRB13190, is a a recombinant rabbit monoclonal antibody that specifically detects Brain lipid-binding protein (BLBP) and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected BLBP/FABP7 in rat brain, human brain, and His-tagged recombinant FABP 2,3,4,7. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected BLBP/FABP7 in human brain tissue sections. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected BLBP/FABP7 in human cerebellum tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | GST/His-tagged full-length recombinant human Brain lipid-binding protein. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 7I23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Brain lipid-binding protein (BLBP). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Fatty acid-binding protein, brain (UniProt: O15540; also known as Brain lipid-binding protein, BLBP, Brain-type fatty acid-binding protein, B-FABP, Fatty acid-binding protein 7, Mammary-derived growth inhibitor related) is encoded by the FABP7 (also known as BLBP, FABPB, MRG) gene (Gene ID: 2173) in human. FABP7 is a member of the large family of hydrophobic ligand-binding proteins (FABPs). FABP proteins are involved in lipid metabolism, including uptake and intracellular trafficking of fatty acids and retinoids. They also play a role in gene regulation, cell signaling, cell growth, and differentiation. FABP7 is a brain-specific protein that is required for the establishment of the radial glial fiber system in the developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers. It is expressed at high levels in the developing central nervous system. It is shown to be important in early nervous system development and is expressed in astrocytes and neuronal cell precursors in mature brain. FABP7 expression is reported to be higher during the light period and lower during the dark in brain regions involved in sleep and activity mechanisms. It is found in melanocytic lesions and has shown to regulate proliferation and invasion in melanoma cells in vitro, suggesting that it may play a role in cell proliferation and invasion. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gerstner, JR et al. (2008). PLoS One 3(2); e1631). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |