Anti-LONP1 Antibody, clone 1I18, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1314 - recombinant, expressed in HEK 293 cells
Synonym: EC:3.4.21.53; LONHs; LONP; Lon protease homolog mitochondrial; Lon protease-like protein; Mitochondrial ATP-dependent protease Lon; Serine protease 15
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human small intestine (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in glandular cells of human small intestine tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human small intestine (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in glandular cells of human small intestine tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human small intestine (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in glandular cells of human small intestine tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and rat colon (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in glandular cells of rat colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and rat colon (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in glandular cells of rat colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and rat colon (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in glandular cells of rat colon tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of MCF-7 cells was performed using a 1:100 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of MCF-7 cells was performed using a 1:100 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of MCF-7 cells was performed using a 1:100 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of HeLa cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of HeLa cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of HeLa cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of A431 cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of A431 cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of A431 cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of HUVEC cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of HUVEC cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of HUVEC cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of NIH3T3 cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of NIH3T3 cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorscent analysis of NIH3T3 cells was performed using a 1:10 dilution of Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Mitochondria.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from A431 (Lane 1), HEK293 (Lane 2), NIH3T3 (Lane 3), and L6 (Lane 4) cells were probed with Cat. No. ZRB1314, Anti-LONP1, clone 1I18 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates LONP1 (~106 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 1I18, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 106.49 kDa |
| observed mol wt ~106 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, mouse, human |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P36776  |
| Application: | Anti-LONP1, clone 1I18 ZooMAb, Cat. No. ZRB1314, is a recombinant rabbit monoclonal antibody that specifically detects mitochondrial Lon protease homolog (LONP1) and has been tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LONP1 in A431, HEK293, and L6 cell lysates. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected LONP1 in human small intestine and rat colon tissue sections. Immunocytochemistry Analysis: A 1:10-100 dilution from a representative lot detected LONP1 in HeLa, A431, HUVEC, NIH 3T3 and MCF-7 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | GST/His-tagged recombinant fragment corresponding to 149 amino acids from the central region of Human LONP1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1I18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Mitochondrial ATP-dependent protease Lon (LONP). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Lon protease homolog, mitochondrial (UniProt: P36776; also known as EC:3.4.21.53, LONHs, Lon protease-like protein, LONP, Mitochondrial ATP-dependent protease Lon, Serine protease 15) is encoded by the LONP1 (also known as PRSS15) gene (Gene ID: 9361) in human. LONP is a homohexameric, nuclear encoded, mitochondrial matrix protein that acts as an ATP-dependent serine protease. It is expressed ubiquitously and is present at high levels in the liver, brain, heart, skeletal muscle, placenta. It is synthesized with a transit peptide (aa 1-67) that helps in its import into the matrix where it is cleaved off to generate the mature form. It is organized in a ring with a central cavity and the ATP-binding and proteolytic domains (AP-domain) form a hexameric chamber. Its N-terminal domain (aa 124-368) is crucial for the overall structure of the protein, maintaining it in a conformation allowing its proper functioning. It is highly conserved throughout evolution and is shown to be crucial for the maintenance of mitochondrial homeostasis. LONP mediates the selective degradation of mutant and abnormal proteins in mitochondria. It also has a chaperone function in the assembly of inner membrane protein complexes. It also participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial DNA. Its DNA and RNA binding is stimulated by substrate and inhibited by ATP binding. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pinti, M., et al. (2016). Biochim. Biophys. Acta 1857(8); 1300-1306). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |