Anti-FCGR2B/CD32 Antibody, clone 3G17 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1276 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1276, Anti-FCGR2B/CD32, clone 3G17 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in trophoblasts of human placenta sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1276, Anti-FCGR2B/CD32, clone 3G17 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in trophoblasts of human placenta sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1276, Anti-FCGR2B/CD32, clone 3G17 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in trophoblasts of human placenta sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of K562 cells was performed using a 1:1,000 dilution of Cat. No. ZRB1276, Anti-FCGR2B/CD32, clone 3G17 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane receptor.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of K562 cells was performed using a 1:1,000 dilution of Cat. No. ZRB1276, Anti-FCGR2B/CD32, clone 3G17 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane receptor.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of K562 cells was performed using a 1:1,000 dilution of Cat. No. ZRB1276, Anti-FCGR2B/CD32, clone 3G17 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane receptor.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from K562 cells (Left Lane) and Human peripheral blood mononuclear cell (PBMC)(Right Lane) were probed with Cat. No. ZRB1276, Anti-FCGR2B/CD32, clone 3G17 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates FCGR2B/CD32 (~35-40 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or FCGR2B/CD32, peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1276, Anti-FCGR2B/CD32, clone 3G17 ZooMAb® Rabbit Monoclonal. The binding of this antibody to FCGR2B/CD32, peptide displayed a KD of 1.5 x 10-7 whereas binding to non-specific control peptide displayed a KD of 2.9 x 10-3.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million K562 cells was performed using 0.1 μg of Cat. No. ZRB1276, Anti-FCGR2B/CD32, clone 3G17 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit IgG secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 3G17, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminus |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 34.04 and 35.58 kDa |
| observed mol wt ~35 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_003992.3  |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P31994 |
| Application: | Quality Control Testing Evaluated by Western Blotting in K562 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FCGR2B/CD32 in K562 cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected FCGR2B/CD32 in human peripheral blood mononuclear cell (PBMC) lysate. Affinity Binding Assay: A representative lot of this antibody bound FCGR2B/CD32 peptides with a KD of 1.5 x 10-7 in an affinity binding assay. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected FCGR2B/CD32 in human placenta tissue sections. Flow Cytometry Analysis: 0.1 μg from a representative lot detected FCGR2B/CD32 in one million K562 cells. Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected FCGR2B/CD32 in K562 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-FCGR2B/CD32, clone 3G17 ZooMAb®, Cat. No. ZRB1276, is a recombinant Rabbit monoclonal antibody that targets FCGR2b-c/CD32 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry & Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal, extracellular domain of human Low affinity immunoglobulin g Fc region receptor II-b/c (CD32). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3G17 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Low affinity immunoglobulin g Fc region receptor II-b/c (CD32). It targets an epitope within 17 amino acids from the N-terminal, extracellular domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Low affinity immunoglobulin g Fc region receptor II-b/c (UniProt: P31994/ P31995; also known as IgG Fc receptor II-b/c, CDw32, Fc-gamma RII-b/c, Fc-gamma-RIIb-c, FcRII-b/c, CD32) is encoded by the FCGR2B/C (also known as CD32, FCG2, IGFR2) gene (Gene ID: 2213/9103) in human. FcRII-b/c are single-pass type 1 membrane proteins that are synthesized with a signal peptide (aa 1-42), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 43-217 for FcRII-b and 43-223 for FcRII-c), a transmembrane domain (aa 218-240 for FcRII-b and 224-246 for FcRII-c) and a cytoplasmic domain (aa 241-310 for FcRII-b and 247-323 for FcRII-c). They also contain two Ig-like C2 domains and an ITIM motif, which is involved in triggering cell activation upon receptors aggregation. This motif can bind the SH2 domain of several SH2-containing phosphatases. FcRII-b/c can undergo phosphorylation by Src-type tyrosine kinases, such as LYN and BLK. This IgG Fc receptor is the most broadly distributed Fc-g receptor and is reported to be expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). However, it is not detected in natural killer cells. It is involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |