Anti-Na+-K+ ATPase α1 Antibody, clone 1E13 ZooMAb^® Rabbit Monoclonal

SIGMA/ZRB1266 - recombinant, expressed in HEK 293 cells

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-ZRB1266-25UL	25 µL	$223.00 1/EA	Add To Favorites
45-ZRB1266-4X25UL	25 µL	$460.00 1/EA	Add To Favorites

Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1266, Anti-Na+-K+ ATPase α1, clone 1E13 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glandular cells and lamina propria of human colon sections.

Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A549 cells was performed using a 1:100 dilution of Cat. No. ZRB1266, Anti-Na+-K+ ATPase α1, clone 1E13 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Membrane.

Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from A431 (Lane 1), MCF-7 (Lane 2), A549 (Lane 3) cells and Human Colon tissue (Lane 4) were probed with Cat. No. ZRB1266, Anti-Na+-K+ ATPase α1, clone 1E13 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Na+-K+ ATPase α1 (~100 kDa).

12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.

12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.

12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.

Properties
Descriptions
Safety Info.
Basic Data

antibody form	purified antibody
antibody product type	primary antibodies
biological source	rabbit
clone	1E13, monoclonal
	recombinant monoclonal
conjugate	unconjugated
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation
form	lyophilized
greener alternative category	Aligned
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry .
isotype	IgG
mol wt	calculated mol wt ~112.90 kDa
	observed mol wt ~100 kDa
packaging	antibody small pack of 25 μL
product line	ZooMAb^® learn more
Quality Level	200
recombinant	expressed in HEK 293 cells
shipped in	ambient
species reactivity	human
species reactivity (predicted by homology)	bovine, rat, monkey, mouse
storage temp.	2-8°C
technique(s)	immunocytochemistry: suitable
	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
	western blot: suitable
UniProt accession no.	P05023

Application:	Quality Control Testing Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Na+-K+ ATPase a1 in A431 cell lysate. Tested Applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Na+-K+ ATPase 1 in MCF-7, A549. and Human colon lysates. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Na+-K+ ATPase 1 in A549 cell line. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Na+-K+ ATPase 1 in human colon tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Application:	Anti-Na+-K+ ATPase a1, clone 1E13 ZooMAb^®, Cat. No. ZRB1266, is a rabbit recombinant monoclonal antibody that specifically targets of Na+-K+ ATPase a1 and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
General description:	ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Immunogen:	KLH-conjugated linear peptide corresponding to 16 amino acids from the third cytoplasmic domain from the N-terminal half of human Na(+)/K(+) ATPase a1 subunit.
Legal Information:	ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form:	Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution:	300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Specificity:	Clone 1E13 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Na(+)/K(+) ATPase a1 subunit. It targets an epitope within 16 amino acids from the third cytoplasmic domain from the N-terminal half.
Storage and Stability:	Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Target description:	Sodium/potassium-transporting ATPase subunit alpha-1 (UniProt: P05023; also known as EC:7.2.2.13, Na (+)/K (+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1) is encoded by the ATP1A1 gene (Gene ID: 476) in human. Na (+)/K (+) ATPase alpha-1 is a multi-pass membrane protein with five extracellular domains; ten helical domains, and six cytoplasmic domains. It is synthesized with a short propeptide (aa 1-5), which is removed in the mature form. It serves as the catalytic component of the active enzyme that catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions that provides energy for active transport of various nutrients. This gradient is also essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. Phosphorylation of Na (+)/K (+) ATPase alpha-1 on tyrosine 10 modulates its pumping activity and phosphorylation on serine 943 by protein kinase A modulates its response protein kinase C. Following increase in intracellular sodium, it undergoes dephosphorylation by protein phosphatase 2A (PP2A) that leads to increase in its catalytic activity. Mutations in ATP1A1 gene have been linked to Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.

2-8°C

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