Anti-Red/Green Opsin Antibody, clone 1D2 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1265 - recombinant, expressed in HEK 293 cells
Synonym: Long-wave-sensitive opsin 1/
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human retina (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1265, Anti-Red/Green Opsin, clone 1D2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in cones within photoreceptor layer of human retina tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human retina (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1265, Anti-Red/Green Opsin, clone 1D2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in cones within photoreceptor layer of human retina tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human retina (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1265, Anti-Red/Green Opsin, clone 1D2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in cones within photoreceptor layer of human retina tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Y79 cells was performed using a 1:100 dilution of Cat. No. ZRB1265, Anti-Red/Green Opsin, clone 1D2 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane protein.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Y79 cells was performed using a 1:100 dilution of Cat. No. ZRB1265, Anti-Red/Green Opsin, clone 1D2 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane protein.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Y79 cells was performed using a 1:100 dilution of Cat. No. ZRB1265, Anti-Red/Green Opsin, clone 1D2 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane protein.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Calf Retina tissue lysate was probed with Cat. No. ZRB1265, Anti-Red/Green Opsin, clone 1D2 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Red/Green Opsin (~36 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Green/Red Opsin peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1265, Anti-Red/Green Opsin, clone 1D2 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Green/Red Opsin peptide displayed a KD of 3.4 x 10-7, whereas binding to non-specific control peptide displayed a KD of 2.3 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1D2, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 40.57 kDa |
| observed mol wt ~36 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_000504.1  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, bovine |
| species reactivity (predicted by homology) | monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P04000 (red) + P04001 (green) |
| Application: | Quality Control Testing Evaluated by Western Blotting in Calf Retina tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Red/Green Opsin in Calf Retina tissue lysate. Tested applications Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Red/Green Opsin in Y79 cells. Affinity Binding Assay: A representative lot of this antibody bound Green/Red Opsin with a KD of 3.4 x 10-7 in an affinity binding assay. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Red/Green Opsin in human retina tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to a sequence of 19 amino acids from the N-terminal, extracellular domain that is common to human Red and Green opsin 1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1D2 is a Rabbit recombinant monoclonal antibody that detects Red/Green opsin. It targets an epitope within 19 amino acids from the first extracellular domain within the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Long-wave-sensitive opsin 1/Medium-wave-sensitive opsin 1 (UniProt: P04000/P04001; also known as Red cone photoreceptor pigment/Green cone photoreceptor pigment, Red-sensitive opsin/ Green-sensitive opsin, ROP/GOP) are encoded by the OPN1LW/OPN1MW (also known as RCP/GCP) genes (Gene ID: 5956/2652) in human. The full range of color discrimination in humans is based on the presence and function of three cone photoreceptors. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein that gives sensitivity in the short (S cone, peak sensitivity about 420 nm), middle (M cone, peak sensitivity about 530 nm with polymorphism), and long (L cone, peak sensitivity about 560 nm with polymorphism) wavelengths of the light spectrum. Opsins are multi-pass membrane proteins that belongs to the G-protein coupled receptor 1 family. They consist of four extracellular, 7 helical, and four cytoplasmic domains. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event. The S cone (blue) opsin seems to have a stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments. Mutations in OPN1MW and OPN1LW genes are known to cause color blindness that is characterized by a dichromasy in which red and green are confused, without loss of luminance or shift or shortening of the spectrum. Some mutations also lead to cone dystrophy leading to progressive degeneration of the cone photoreceptor with some preservation of rod function. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Neitz, M., and Neitz, J. (2000). Arch. Ophthalmol. 118(5); 691-700). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |