Anti-Reelin Antibody, clone 3F13 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1264 - recombinant, expressed in HEK 293 cells
Synonym: RELN
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Cerebral Cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons of Human Cerebral Cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Cerebral Cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons of Human Cerebral Cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Cerebral Cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons of Human Cerebral Cortex tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of C6 cells was performed using a 1:100 dilution of Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the extracellular matrix.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of C6 cells was performed using a 1:100 dilution of Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the extracellular matrix.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of C6 cells was performed using a 1:100 dilution of Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the extracellular matrix.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal immunofluorescent analysis of Human Cerebral Cortex tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the extracellular matrix.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal immunofluorescent analysis of Human Cerebral Cortex tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the extracellular matrix.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal immunofluorescent analysis of Human Cerebral Cortex tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the extracellular matrix.
Western Blotting Enhanced Validation - Recombinant Antibody Technology HepG2 cell lysate were probed with Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Reelin (~380 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 10 mg/mL of non-specific GST protein (Run 1) or recombinant Reelin protein fragment (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1264, Anti-Reelin, clone 3F13 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Reelin protein fragment displayed a KD of 2.3 x 10-8, whereas binding to non-specific GST protein displayed a KD of 2.0 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 3F13, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminal half |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 388.39 kDa |
| observed mol wt ~380 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_005036  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P78509 |
| Application: | Quality Control Testing Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Reelin in HepG2 cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Reelin in C6 cell lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Reelin in human cerebral cortex tissue sections. Affinity Binding Assay: A representative lot of this antibody bound Reelin with a KD of 2.3 x 10-8 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Reelin in C6 cells. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Reelin in human cerebral cortex tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | His-tagged recombinant fragment corresponding to 333 amino acids from the N-terminal half of human Reelin. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3F13 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Reelin. It targets an epitope within the N-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Reelin (UniProt: P78509; also known as RELN) is encoded by the RELN gene (Gene ID: 5649) in human. Reelin is a large extracellular matrix glycoprotein with serine protease activity. It is synthesized with a signal peptide (aa 1-25) that is subsequently cleaved off to produce the mature form that contains 8 EGF-like domains. Its Reelin domain is located in amino acids 26-190. Reelin plays a role in brain development and layering of neurons in the cerebral cortex and cerebellum. It is abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In the adult brain it is preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. It is also expressed in the retina, liver, thyroid gland, adrenal gland, fallopian tube, and breast tissue. Reelin is shown to regulate the positioning of neurons and participates in neurotransmission and memory formation. It also regulates microtubule function in neurons and neuronal migration and affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Reelin participates in canonical signaling via binding to VLDL receptor and apolipoprotein E receptor 2 and induces tyrosine phosphorylation of the adaptor protein disabled 1 (Dab1) by Src kinases. Phosphorylated Dab1 in-turn activates downstream signaling, including the PI3-kinase-dependent pathways. Mutations in RELN gene have been linked to familial temporal lobe epilepsy and Lissencephaly that is associated with ataxia, mental retardation, and seizures. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mikulska-Ruminska, K., et al. (2019). Sci. Rep. 9; 18974; Dlugosz, P. and Nimpf, J. (2018). J. Mol. Sci. 19(10); E3090). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |