Anti-Podocin Antibody, clone 1C10 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1262 - recombinant, expressed in HEK 293 cells
Synonym: Nephrosis 2 idiopathic steroid-resistant
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1262, Anti-Podocin, clone 1C10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glomeruli of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1262, Anti-Podocin, clone 1C10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glomeruli of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1262, Anti-Podocin, clone 1C10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glomeruli of human kidney tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human kidney tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1262, Anti-Podocin, clone 1C10 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane and endoplasmic reticulum.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human kidney tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1262, Anti-Podocin, clone 1C10 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane and endoplasmic reticulum.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human kidney tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1262, Anti-Podocin, clone 1C10 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane and endoplasmic reticulum.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Human Kidney membrane lysate was probed with Cat. No. ZRB1262, Anti-Podocin, clone 1C10 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Podocin (~42 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Podocin peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1262, Anti-Podocin, clone 1C10 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Podocin peptide displayed a KD of 4.1 x 10-8, whereas binding to non-specific control peptide displayed a KD of 3.2 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1C10, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 42.2 kDa |
| observed mol wt ~42 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_055440.1  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9NP85 |
| Application: | Quality Control Testing Evaluated by Western Blotting in Human kidney tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Podocin in Human kidney tissue lysate. Tested applications Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Podocin in human kidney tissue sections. Affinity Binding Assay: A representative lot of this antibody bound Podocin peptide with a KD of 4.1 x 10-8 in an affinity binding assay. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Podocin in human kidney tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Podocin. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1C10 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Podocin. It targets an epitope within 19 amino acids from the C-terminal, cytoplasmic domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Podocin (UniProt: Q9NP85; also known as NPHS2) is encoded by the NPHS2 gene (Gene ID: 7827) in human. Podocin is a member of the stomatin family, which consists of hairpin-like integral membrane proteins with intracellular NH2- and COOH-termini. It is exclusively expressed in the podocytes of fetal and mature kidney glomeruli. It is a raft associated component of the glomerular foot-process membrane where it is localized at the insertion of the slit-diaphragm in podocytes. It plays a role in the regulation of glomerular permeability, acting as a linker between the plasma membrane and the cytoskeleton. Two isoforms of Podocin have been described that are produced by alternative splicing. Isoform 1 is located in cell membrane and peripheral membranes and isoform 2 is present in endoplasmic reticulum. Knock-out mice that lack slit diaphragm die within the first weeks of life due to renal failure. Mutations in NPHS2 gene are linked to the development of a steroid resistant nephrotic syndrome (NPHS2) that is characterized by severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia, edema, and end-stage renal failure in the first two decades of life. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Volker, LA., et al. (2013). BMC Nephrology. 14; Article number: 102; Caridi, G., et al. (2005). Pediatric Res. 57(5 pt2); 54-61). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |