Anti-Mn-SOD Antibody, clone 1C14 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1246 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1246, Anti-Mn-SOD, clone 1C14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in glandular cells and lamina propria of human colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1246, Anti-Mn-SOD, clone 1C14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in glandular cells and lamina propria of human colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1246, Anti-Mn-SOD, clone 1C14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in glandular cells and lamina propria of human colon tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:1,000 dilution of Cat. No. ZRB1246, Anti-Mn-SOD, clone 1C14 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:1,000 dilution of Cat. No. ZRB1246, Anti-Mn-SOD, clone 1C14 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:1,000 dilution of Cat. No. ZRB1246, Anti-Mn-SOD, clone 1C14 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the mitochondria.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from HeLa cells (Lane 1) and HepG2 cells (Lane 2) were probed with Cat. No. ZRB1246, Anti-Mn-SOD, clone 1C14 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Mn-SOD (~25 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific GST protein (Run 1) or recombinant Mn-SOD protein (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1246, Anti-Mn-SOD, clone 1C14 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Mn-SOD protein displayed a KD of 1.5 x 10-6, whereas binding to non-specific GST protein displayed a KD of 3.9 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1C14, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt ~24.75 kDa |
| observed mol wt ~25 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, human |
| species reactivity (predicted by homology) | monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P04179  |
| Application: | Quality Control Testing Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mn-SOD in HepG2 cell lysate. Tested Applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Mn-SOD in HeLa cell lysate. Affinity Binding Assay:: A representative lot of this antibody bound Mn-SOD proteins with a KD of 4.5 x 10-8 in an affinity binding assay. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Mn-SOD in human colon tissue sections. Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Mn-SOD in PC12 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Mn-SOD, clone 1C14 ZooMAb®, Cat. No. ZRB1246, is a recombinant Rabbit monoclonal antibody that specifically targets Mn-SOD and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | His-tagged full-length mature (without the transit peptide) recombinant human Mn-SOD. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1C14 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human Mn-SOD. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Superoxide dismutase [Mn], mitochondrial (UniProt: P04179; also known as EC:1.15.1.1, Mn-SOD) is encoded by the SOD2 gene (Gene ID: 6648) in human. Superoxide dismutases (SODs) provide defense against reactive oxygen species. Three types of SODs are found in organisms and are named based on the nature of the metal ion(s) found at their active sites. The Cu/Zn superoxide dismutase (Cu/ZnSOD), is primarily localized in the cytosol, nucleus, peroxisome, and intermembrane of mitochondria in eukaryotes. The Mn-SOD is found in the mitochondria, and the Fe-SOD is found primarily in bacteria. Mn-SOD is a homotetrameric enzyme, localized in the mitochondrial matrix, catalyzes the dismutation of superoxide radicals in mitochondria by converting anion superoxide into hydrogen peroxide and oxygen. Each monomer of Mn-SOD contains an active site bound to manganese (Mn) ion. Mn-SOD constitutes about 10 15% of the total SODs. The Mn ions can change between its 3+ and 2+ oxidation states within the dismutase mechanism that consists of two half-reactions. Electron exchange in metal complexes is reported to occur by either an inner-sphere or outer-sphere mechanism. In inner-sphere electron transfer, the metal cofactor is oxidized or reduced by a ligand bound directly to the metal, whereas in the outer-sphere mechanism, the metal is oxidized or reduced by a group not directly coordinated to the metal cofactor. The catalysis of superoxide anions by Mn-SOD occurs by an inner-sphere mechanism. Peroxynitrite radical can react with Mn-SOD that can cause a site-specific nitration of the critical tyrosine leading to its inactivation. Four isoforms of Mn-SOD have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Candas, D., and Li, JJ. (2014). Antioxid. Redox Signal. 20(10); 1599-1617). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |