Anti-uPAR Antibody, clone 2G10 , ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1224 - recombinant, expressed in HEK 293 cells
Synonym: CD87; Monocyte activation antigen Mo3; U-PAR; Urokinase plasminogen activator surface receptor
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human lung cancer (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1224, Anti-uPAR, clone 2G10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in areas surrounding tumor cells of human large cell lung cancer sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human lung cancer (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1224, Anti-uPAR, clone 2G10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in areas surrounding tumor cells of human large cell lung cancer sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human lung cancer (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1224, Anti-uPAR, clone 2G10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in areas surrounding tumor cells of human large cell lung cancer sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HT1080 cell line was performed using a 1:100 dilution of Cat. No. ZRB1224, Anti-uPAR, clone 2G10 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HT1080 cell line was performed using a 1:100 dilution of Cat. No. ZRB1224, Anti-uPAR, clone 2G10 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HT1080 cell line was performed using a 1:100 dilution of Cat. No. ZRB1224, Anti-uPAR, clone 2G10 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Unreduced uPAR (Lane 1) and uPAR reduced (Lane 2) were probed with Cat. No. ZRB1224, Anti-uPAR, clone 2G10 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates uPAR (~45 kD for non-reduced recomb protein).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 2G10, recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | observed mol wt ~45 kDa |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| inhibition assay: suitable (peptide) | |
| western blot: suitable | |
| UniProt accession no. | Q03405  |
| Application: | Anti-uPAR, clone 2G10 ZooMAb, Cat. No. ZRB1224, is a Rabbit monoclonal antibody that targets uPAR and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Inhibition Assay, and Western Blotting. |
| Application: | Inhibits Activity/Function: A representative lot of this antibody inhibited the invasion of MDA-MB-231 cells (Harel, E., et al., 2014 UCSF Scientific Poster). Flow Cytometry Analysis: 0.1 μg from a representative lot detected uPAR in one million SH-SY5Y cells. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected uPAR in human lung cancer tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected uPAR in HT1080 cells. Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | A recombinant fragment corresponding the first 277 amino acids from human Urokinase plasminogen activator surface receptor (uPAR). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2G10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Urokinase plasminogen activator surface receptor (uPAR). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Urokinase plasminogen activator surface receptor (UniProt: Q03405; also known as U-PAR, uPAR. Monocyte activation antigen Mo3, CD87) is encoded by the PLAUR (also known as MO3, UPAR) gene (Gene ID: 5329) in human. uPAR is a GPI-anchored cell membrane receptor for urokinase plasminogen activator (uPA), an enzyme responsible for the activation of plasmin and degradation of extracellular matrix components. It is also a subject to negative-feedback regulation by U-PA, which cleaves it into an inactive form. uPAR is synthesized with a signal peptide (aa 1-22) and a propeptide (aa 306-335), which are subsequently cleaved off to produce the mature form. The mature form has three homologous domains known as DI, DII, and DIII. Higher expression of uPAR has been reported in many human cancers, including non-small cell lung cancer and colorectal cancer and it correlates with a poor prognosis and early invasion and metastasis. uPAR, through integrins interaction, is reported to play a crucial role in activating Src, FAK and paxillin, which are considered as critical mediators of cell migration. In addition, via its interaction with chemotaxis receptors for fMet-lleu-Phe, it mediates both uPA and fMLF-dependent cell migration. uPAR has also been implicated in cross-talk with EGFR, which mediates the shift from tumor cell dormancy to proliferation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Di Mauro, C., et al. (2017). Sci. Rep. 7; Article 9388). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |