Anti-Beclin 1 Antibody, clone 1E22 , ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1222 - recombinant, expressed in HEK 293 cells
Synonym: Coiled-coil myosin-like BCL2-interacting protein; Protein GT197
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1222, Anti-Beclin 1, clone 1E22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/punctate cytoplasmic staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1222, Anti-Beclin 1, clone 1E22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/punctate cytoplasmic staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1222, Anti-Beclin 1, clone 1E22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/punctate cytoplasmic staining was observed in human breast cancer tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cell line treated wtih Chloroquine (100 μM for 3 h) was performed using a 1:1,000 dilution of Cat. No. ZRB1222, Anti-Beclin 1, clone 1E22 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the punctate cytoplasmic staining in treated cells.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cell line treated wtih Chloroquine (100 μM for 3 h) was performed using a 1:1,000 dilution of Cat. No. ZRB1222, Anti-Beclin 1, clone 1E22 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the punctate cytoplasmic staining in treated cells.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cell line treated wtih Chloroquine (100 μM for 3 h) was performed using a 1:1,000 dilution of Cat. No. ZRB1222, Anti-Beclin 1, clone 1E22 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the punctate cytoplasmic staining in treated cells.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysate from HeLa cells treated with Chloroquine (100 μM for 3 h) (Lane 1) and untreated HeLa (Lane 2) were probed with Cat. No. ZRB1222, Anti-Beclin 1, clone 1E22 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Beclin-1 (~58 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 1E22, recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 51.9 kDa |
| observed mol wt ~58 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q14457  |
| Application: | Anti-Beclin 1, clone 1E22 ZooMAb, Cat. No. ZRB1222, is a highly specific rabbit recombinant monoclonal antibody that targets Beclin-1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Beclin-1 in human breast cancer tissue sections. Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Beclin-1 in HeLa cells treated with Chloroquine (100 uM for 3 h). Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human Beclin-1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1E22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Beclin-1. It targets an epitope within 24 amino acids from the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Beclin-1 (UniProt: Q14457; also known as Coiled-coil myosin-like BCL2-interacting protein, Protein GT197) is encoded by the BECN1 (also known as GT197) gene (Gene ID: 8678) in human. Beclin-1 is a peripheral membrane protein that plays a key role in autophagy. It is a component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and Beclin-1 associating with additional regulatory/auxiliary subunits to form alternative complex forms. It interacts with several cofactors, such as Atg14L, UVRAG, Rubicon, Ambra1, and survivin to regulate the activity of phosphatidylinositol 3-kinase. Its coiled-coil domain (aa 142-270) can form antiparallel homodimers and mediate dimerization with the coiled coil domains of ATG14 or UVRAG. Baclin-1 contains a BH3 motif (aa 108-127) that is shown to be bound to and inhibited by Bcl-2 or Bcl-xL. It also contains a C-terminal evolutionary conserved domain (ECD; aa 245-450) that has poly-Gln-binding domains and within this ECD it has a short amino acid sequence (aa 425-450) that is required for its membrane association. Beclin can undergo cleavage into Beclin-1-C (35 kDa; aa 134-450) and Beclin-1-C (37 kDa; aa 150-450). The Beclin-1-C 35 kDa localizes to mitochondria and can promote apoptosis by inducing the mitochondrial translocation of Bax and the release of proapoptotic factor. Beclin-1 is shown to undergo caspase-mediated cleavage that promotes crosstalk between apoptosis and autophagy. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |