Anti-Mitofusin-2 Antibody, clone 2D19 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1202 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cells was performed using a 1:100 dilution of Cat. No. ZRB1202, Anti-Mitofusin-2, clone 2D19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the perinuclear and/or punctate cytoplasmic staining.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cells was performed using a 1:100 dilution of Cat. No. ZRB1202, Anti-Mitofusin-2, clone 2D19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the perinuclear and/or punctate cytoplasmic staining.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cells was performed using a 1:100 dilution of Cat. No. ZRB1202, Anti-Mitofusin-2, clone 2D19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the perinuclear and/or punctate cytoplasmic staining.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from HeLa (Lane 1), U2OS (Lane 2), COS-7 (Lane 3), and PC12 (Lane 4) cells were probed with Cat. No. ZRB1202, Anti-Mitofusin-2, clone 2D19 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Mitofusin-2 (~79 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Mitofusin-2 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1202, Anti-Mitofusin-2, clone 2D19 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Mitofusin-2 peptide displayed a KD of 1.4 x 10-7, whereas binding to non-specific control peptide displayed a KD of 1.2 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2D19, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt ~86.4 kDa |
| observed mol wt ~79 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | monkey, rat, human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | O95140  |
| Application: | Quality Control Testing Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mitofusin-2 in HeLa cell lysate. Tested Applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Mitofusin-2 in U2OS, COS-7, and PC12 cell lysates. Immunocytochemistry Analysis: A 1:100 dilution from a representative detected Mitofusin-2 in HeLa cells. Affinity Binding Assay: A representative lot of this antibody bound Mitofusin-2 peptides with a KD of 1.4 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Mitofusin-2, clone 2D19 ZooMAb®, Cat. No. ZRB1202, is a recombinant Rabbit monoclonal antibody that specifically targets Mitofusin-2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 21 amino acids from the first cytoplasmic domain in the C-terminal region of human Mitofusin-2. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2D19 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Mitofusin-2. It targets an epitope within 21 amino acids from the cytoplasmic domain in the C-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Mitofusin-2 (UniProt: O95140; also known as Transmembrane GTPase MFN2) is encoded by the MFN2 (also known as CPRP1, KIAA0214) gene (Gene ID: 9927) in human. Mitofusin-1 and 2 are highly homologous mitochondrial proteins that display ~80% similarity in humans. Mitofusin-2 is a multi-pass membrane protein that serves as a mitochondrial outer membrane GTPase to mediate mitochondrial clustering and fusion. It is ubiquitously expressed at low level, but higher expression is observed in heart and kidney. It consists of a large, cytoplasmic N-terminal GTPase domain, followed by a spacer, a first coiled-coil heptad-repeat (HR1) domain, a spacer, two transmembrane domains that cross the outer mitochondrial membrane, a spacer and a second, C-terminal heptad-repeat domain (HR2). Between HR1 and transmembrane domains, it contains a proline-rich domain that participates in protein protein interactions. Mitofusin-2 plays a key role in mitochondrial metabolism and is involved in mitochondrial fusion, trafficking, turnover, contacts with other organelles, and in the clearance of damaged mitochondria via selective mitophagy. Mitofusin-2 is phosphorylated by PINK1 and the phosphorylated form functions as a receptor for parkin that mediates its ubiquitination, a signal to mark damaged mitochondria. It is also required in the control of unfolded protein response upon endoplasmic reticulum stress. Mitofusion-2 knockout cells display mitochondrial spheres or ovals of different sizes and are several folds larger in diameter than wild-type. Mutations in MFN2 gene are known to cause Charcot-Marie-Tooth disease that is characterized by signs of axonal degeneration and progressive distal muscle weakness and atrophy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Filadi, R., et al. (2018). Cell Death Dis. 9; Article 330). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |