Anti-LDL Receptor Antibody, clone 2N19, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1176 - recombinant, expressed in HEK 293 cells
Synonym: Anti-FH; Anti-FHC; Anti-FHCL1; Anti-LDLCQ2
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human liver (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in ductal cells & sinusoids of human liver sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human liver (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in ductal cells & sinusoids of human liver sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human liver (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in ductal cells & sinusoids of human liver sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and rat liver (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Membranous/ECM staining was observed in subsets of hepatocytes throughout rat liver sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and rat liver (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Membranous/ECM staining was observed in subsets of hepatocytes throughout rat liver sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and rat liver (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Membranous/ECM staining was observed in subsets of hepatocytes throughout rat liver sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of A431 cell line was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Endosome, lysosome, Golgi, cell membrane, secreted.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of A431 cell line was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Endosome, lysosome, Golgi, cell membrane, secreted.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of A431 cell line was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Endosome, lysosome, Golgi, cell membrane, secreted.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HEPG2 cell line was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Endosome, lysosome, Golgi, cell membrane, secreted.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HEPG2 cell line was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Endosome, lysosome, Golgi, cell membrane, secreted.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HEPG2 cell line was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Endosome, lysosome, Golgi, cell membrane, secreted.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH-3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Endosome, lysosome, Golgi, cell membrane, secreted.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH-3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Endosome, lysosome, Golgi, cell membrane, secreted.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH-3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Endosome, lysosome, Golgi, cell membrane, secreted.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Recombinant human LDL Receptor was probed with Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Lanes 1: Human LDL receptor at 100 ng Lane 2: Human LDL receptor at 10 ng Lane 3: Human LDL receptor at 1 ng Arrow indicates LDL Receptor (~100 kDa)
Western Blotting Enhanced Validation-Recombinant Antibody Technology RAW264.7 (A) and HEPG2 (B) lysates were probed with Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates LDL Receptor (~100 kDa).
Flow Cytometry Enhanced Validation-Recombinant Antibody Technology Staining of one million HepG2 cells was performed using 0.1 μg of Cat. No. ZRB1176, Anti-LDL Receptor, clone 2N19 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 2N19, recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 95.38 kDa |
| observed mol wt ~95 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, rat |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| western blot: suitable (peptide) | |
| UniProt accession no. | P01130  |
| Application: | Anti-LDL Receptor, clone 2N19 ZooMAb , Cat. No. ZRB1176, is a highly specific rabbit recombinant monoclonal antibody that targets Low-Density Lipoprotein Receptor and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LDL Receptor in RAW264.7 and HEPG2 cell lysates. Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected LDL Receptor in human liver and rat liver tissue sections. Immunocytochemistry Analysis: A 1:100 dilution of from a representative lot detected LDL Receptor in A431, HepG2, NIH 3T3 cells. Flow Cytometry Analysis: 0.1 μg from a representative lot detected LDL Receptor in HepG2. Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | His-tagged recombinant fragment corresponding to 105 amino acids from the C-terminal region of human LDL receptor. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2N19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects LDL receptor. It targets an epitope within 105 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Low-density lipoprotein receptor (UniProt P01130; also known as LDL receptor) is encoded by the LDLR (also known as FH, FHC) gene (Gene ID: 3949) in human. LDL receptor is a single-pass type I membrane protein that is rapidly endocytosed upon ligand binding. It binds low density lipoprotein (LDL) and transports it into cells by endocytosis. LDL receptor recognizes the apoprotein B100 embedded in the outer phospholipid layer of cholesterol-rich LDL particles and mediates their removal by endocytosis via clathrin-coated pits. It also recognizes the ApoE protein found in chylomicron remnants and VLDL remnants (IDL). LDL receptor is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form. The mature form of LDL receptor contains an extracellular domain (aa 22-788), a transmembrane domain (aa 789-810), and a cytoplasmic domain (aa 811-860). The extracellular region contains seven class A (aa 25-313) and six class B (aa 397-658) LDLR repeats and three EGF-like domains (aa 314-353, 354-393, 663-712). LDL-A repeats are known to mediate LDL binding, and repeats 2-7 are required for ApoB binding, while only repeat 5 is required for ApoE binding. The class B (LDL-B) repeats region adopts a beta-propeller conformation and is believed to be responsible for the pH-dependent release of LDL in the endosome. The cytoplasmic tail contains a signal sequence that is important for localizing the receptors to clathrin-coated pits and for triggering receptor-mediated endocytosis upon LDL binding. Mutations in LDLR gene are known to cause familial hypercholesterolemia (FH) leading to high levels of serum LDL and excess deposition of cholesterol in tissues leading to atherosclerosis and higher risk of premature coronary heart disease. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |