Anti-phospho JNK (Thr183, Tyr185) Antibody, clone 3F7, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1173 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human colon cancer (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb®, Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in human colon cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human colon cancer (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb®, Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in human colon cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human colon cancer (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb®, Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in human colon cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human ovarian cancer (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb®, Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/nuclear staining was observed in human ovarian cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human ovarian cancer (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb®, Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/nuclear staining was observed in human ovarian cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human ovarian cancer (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb®, Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/nuclear staining was observed in human ovarian cancer tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HeLa cells treated with Anisomycin was performed using a 1:100 dilution of Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HeLa cells treated with Anisomycin was performed using a 1:100 dilution of Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HeLa cells treated with Anisomycin was performed using a 1:100 dilution of Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from NIH-3T3 treated with Anisomyocin (Lane 1) and from untreated NIH-3T3 (Lane 2) cells were probed with Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb®, Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho JNK1/2 (Thr183, Tyr185) (~48 and ~44 kDa)
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from HeLa cells treated with Anisomyocin (Lane 1) and from untreated HeLa (Lane 2) cells were probed with Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb®, Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho JNK 1/2(Thr183, Tyr185) (~48 and ~44 kDa)
Peptide Inhibition Enhanced Validation-Recombinant Antibody Technology Lysates from NIH/3T3 cells treated with Anisomycin were probed with Cat. No. ZRB1173, Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminesence detection system.Lanes 1: NIH/3T3 cells treated with Anisomycin 2: NIH/3T3 cells treated with Anisomycin and Modified Peptide 3: NIH/3T3 cells treated with Anisomycin and Control Peptide 4: HeLa cells treated with Anisomycin 5: HeLa cells treated with Anisomycin and Modified Peptide 6: HeLa cells treated with Anisomycin and Control Peptide Arrow indicates phospho JNK 1/2 (Thr183, Tyr185) (~48 kDa and ~44 kDa)
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 3F7, recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 48.29 kDa |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| inhibition assay: suitable (peptide) | |
| western blot: suitable (peptide) | |
| UniProt accession no. | P45983/P45984  |
| Application: | Anti-phospho JNK (Thr183, Tyr185), clone 3F7 ZooMAb , Cat. No. ZRB1173, is a highly specific recombinant rabbit monoclonal antibody that detects JNK/SAPK phosphorylated on Thr183 and Tyr185 and has been tested for use in Immunocytochemistry, Immunohistochemistry, Peptide Inhibition Assay, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho JNK (Thr183, Tyr185) in NIH3T3 cells treated with Anisomyocin. Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected phospho JNK (Thr183, Tyr185) in human colon cancer and human ovarian cancer tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho JNK (Thr183, Tyr185) in HeLa cells treated with Anisomycin treatment. Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot was used with NIH3T3 cells treated with Anisomyocin ((25 ug/ml for 30 min following overnight serum starvation) for peptide block analysis. Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphothreonine 183 and phosphotyrosine 185 from human JNK/SAPK. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3F7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects JNK1/2 phosphorylated on threonine 183 and tyrosine 185. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Mitogen-activated protein kinase 8/9 (UniProt: P45983/P45984; also known as MAP kinase 8/9, MAPK8/9, Stress-activated protein kinase 1c/1a, SAPK1c/SAPK1a, Stress-activated protein kinase JNK1/ JNK2, c-Jun N-terminal kinase 1/2) is encoded by the MAPK8/9 (also known as JNK1/JNK2, PRKM8/9, SAPK1/2, SAPK1C/1A) gene (Gene ID: 5599/ 5601) in human. SAPK/JNK is a serine/threonine-protein kinase that is involved in various biological processes, including cell proliferation, differentiation, migration, transformation, and programmed cell death. It is activated in response to extracellular stimuli such as proinflammatory cytokines or physical stress. Both JNK1 and JNK2 contain a TXY motif (aa 183-185) that contains the threonine and tyrosine residues whose phosphorylation activates these kinases. Protein kinase domain of SAPK/JNK is localized to amino acids 26-321. Phosphorylation and activation of SAPK/JNK is achieved by two dual specificity kinases, MAP2K7 and MAP2K4. Following their activation SAPK/JNK phosphorylate several transcription factors, primarily components of AP-1 such as JUN, JDP2, and ATF2 and thus regulates AP-1 transcriptional activity. It promotes apoptotic cell death in stressed cells by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. The immunogen sequence used to generate this clone is conserved in both JNK1 and JNK2. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |