Anti-S6 Ribosomal Protein Antibody, clone 1E9, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1172 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human pancreas (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/nuclear/perinuclear staining was observed in acinar cells in human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human pancreas (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/nuclear/perinuclear staining was observed in acinar cells in human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human pancreas (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/nuclear/perinuclear staining was observed in acinar cells in human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and mouse pancreas (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/nuclear/perinuclear staining was observed in acinar cells in mouse pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and mouse pancreas (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/nuclear/perinuclear staining was observed in acinar cells in mouse pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and mouse pancreas (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic/nuclear/perinuclear staining was observed in acinar cells in mouse pancreas tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HeLa cell line was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HeLa cell line was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HeLa cell line was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of A431 cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of A431 cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of A431 cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HUVEC was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HUVEC was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HUVEC was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Jurkat cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Jurkat cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Jurkat cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of MCF-7 cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of MCF-7 cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of MCF-7 cells was performed using a 1:100 dilution of Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation-Recombinant Antibody Technology RAW264.7 cell lysate was probed with Cat. No. ZRB1172, Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates S6 Ribosomal Protein (~32 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 1E9, recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| mol wt | calculated mol wt 28.68 kDa |
| observed mol wt ~32 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable (peptide) | |
| UniProt accession no. | P62753  |
| Application: | Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb , Cat. No. ZRB1172, is a highly specific rabbit recombinant monoclonal antibody that targets S6 Ribosomal Protein and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected S6 Ribosomal Protein in human pancreas and mouse pancreas tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S6 Ribosomal Protein in HeLa, A431, HUVEC, NIH 3T3, Jurkat, MCF-7 cells. Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | His-tagged recombinant fragment corresponding to 136 amino acids from the N-terminal region of human 40S Ribosomal Protein S6. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1E9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects 40S ribosomal protein S6. It targets an epitope within the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | 40S ribosomal protein S6 (UniProt: P62753; also known Phosphoprotein NP33, Small ribosomal subunit protein eS6; RpS6) is encoded by the RPS6 (also known as OK/SW-cl.2) gene (Gene ID: 6194) in human. RpS6 is a highly conserved member of the eukaryotic ribosomal protein eS6 family and is a component of the 40S ribosomal subunit. It plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. It is the major substrate of protein kinases in eukaryote ribosomes and its phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. RpS6 can be phosphorylated on five serine residues (Ser235, 236, 240, 244, and 247) in the region close to the carboxy terminus. Among the serine residues 235 and 236 are shown to be directly phosphorylated by S6Kinase that is subjected to regulation by the nutrient-mTORC1 signaling. Phosphorylation at serine 240/244 is reported to be predominantly nuclear but is also detectable in the cytoplasm, whereas serine 235/236 phosphorylated S6 is almost exclusively localized to the nucleus of primary human cells and is virtually undetectable in the cytoplasm, except in transformed cells where it can also be detected in the cytoplasm. RpS6 is also shown to exist in complex with the Kaposi s sarcoma-associated herpesvirus latency-associated nuclear antigen (LANA). This interaction is mediated by the N-terminal domain of LANA. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rosner, M., et al. (2011). Amino Acids 40(2); 595-600; Chen, W., and Dittmar, DP (2011). J. Virol. 85(18); 9495-9505; Biever, A., et al. (2015). J. Neurosci. 2015, 35(10); 4113-4130) |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |