Anti-Collagen Type III Antibody, clone 3N18 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1167 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skin (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1167, Anti-Collagen Type III, clone 3N18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in collagen present in dermis of human skin tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skin (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1167, Anti-Collagen Type III, clone 3N18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in collagen present in dermis of human skin tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skin (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1167, Anti-Collagen Type III, clone 3N18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in collagen present in dermis of human skin tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology HT1080 cell lysate was probed with Cat. No. ZRB1167, Anti-Collagen Type III, clone 3N18 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Collagen Type III (~200 kDa)
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Collagen Type III peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1167, Anti-Collagen Type III, clone 3N18 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Collagen Type III peptide displayed a KD of 6.7 x 10-6, whereas binding to non-specific control peptide displayed a KD of 3.4 x 10-3.
ELISA Enhanced Validation - Recombinant Antibody Technology 0.1 μg of Collagen Type III peptide and control peptide were analyzed by ELISA using various dilutions (starting at 3 mg/mL) of Cat. No. ZRB1167, Anti-Collagen Type III, clone 3N18 ZooMAb® Rabbit Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Rabbit secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 3N18, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 138.56 kDa |
| observed mol wt ~200 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_000081.1?  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P02461 |
| Application: | Quality Control Testing Evaluated by Western Blotting in HT1080 cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Collagen Type III in HT1080 cell lysate. Tested applications Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Collagen Type III in human skin tissue sections. Affinity Binding Assay: A representative lot of this antibody bound Collagen Type III with a KD of 6.7 x 10-6 in an affinity binding assay. ELISA Analysis: 300 μg/mL from a representative lot detected Collagen Type III in Collagen Type III in an indirect ELISA. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Collagen Type III, clone 3N18 ZooMAb®, Cat. No. ZRB1167, is a recombinant Rabbit monoclonal antibody that detects Collagen Type III and is tested for use in Affinity Binding Assay, ELISA, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human Collagen a1(III) chain. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3N18 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Collagen Type III. It targets an epitope with 22 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Collagen alpha-1(III) chain (UniProt: P02461; also known as Collagen type IIIa) is encoded by the COL3A1 gene (Gene ID: 1281) in human. Collagen is the most abundant protein in the body that is predominantly synthesized by fibroblasts. However, epithelial cells may also synthesize small amounts of collagen. The collagen super family includes over 20 different types of collagen with at least 38 distinct polypeptide chains. Collagen type III occurs in most soft connective tissues along with type I collagen. It is a major structural component in hollow organs such as large blood vessels and uterus that must withstand stretching. Collagen type III is also involved in regulation of cortical development. It is reported to be the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12. It is synthesized with a signal peptide (aa 1-23), an N-terminal propeptide (aa 24-153), and a C-terminal propeptide (aa 1222-1466), which are subsequently cleaved off to generate the mature form. Three identical type III procollagen chains come together in the C-terminal ends to form collagen III molecule and it is stabilized by the formation of disulfide bonds. Each individual chain folds into left-handed helix and the three chains are then wrapped together into a right-handed superhelix, the triple helix. Mutations in COL3A1 are linked to Ehlers-Danlos syndrome, a connective tissue disorder that is characterized by skin hyperextensibility, articular hypermobility, and tissue fragility. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kuivbaniemi, H., and Tromp, G. (2019). Gene. 707; 151-171). |
| Storage Temp. | 2-8°C |