Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human tonsil (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/perinuclear staining was observed both germinal center & non-germinal center cells, as well as epithelium of human tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human tonsil (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/perinuclear staining was observed both germinal center & non-germinal center cells, as well as epithelium of human tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human tonsil (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/perinuclear staining was observed both germinal center & non-germinal center cells, as well as epithelium of human tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and mouse testis (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/perinuclear staining was observed both germinal center & non-germinal center cells, as well as seminiferous tubules & Leydig cells of mouse testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and mouse testis (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/perinuclear staining was observed both germinal center & non-germinal center cells, as well as seminiferous tubules & Leydig cells of mouse testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and mouse testis (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/perinuclear staining was observed both germinal center & non-germinal center cells, as well as seminiferous tubules & Leydig cells of mouse testis tissue sections.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of HeLa cells was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of HeLa cells was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of HeLa cells was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of A431 cells was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of A431 cells was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of A431 cells was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of HUVEC cell line was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of HUVEC cell line was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of HUVEC cell line was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of NIH3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of NIH3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of NIH3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of Jurkat cell line was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Jurkat cell line was performed using a 1:100 dilution of Cat. No. ZRB1147-25UL, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of Jurkat cell line was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of MCF-7 cell line was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of MCF-7 cell line was performed using a 1:100 dilution of Cat. No. ZRB1147-25UL, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry
Enhanced Validation-Recombinant Antibody Technology
Immunofluorescent analysis of MCF-7 cell line was performed using a 1:100 dilution of Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from HeLa (Lane 1), Jurkat (Lane 2), NIH/3T3 (Lane 3), and MCF7 (Lane 4) cells were probed with Cat. No. ZRB1147, Anti-Calnexin, clone 10N19 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Calnexin (~78 kDa).
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Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.