Anti-Calponin 1 Antibody, clone 9D14, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1146 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human uterus (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1146, Anti-Calponin 1, clone 9D14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in smooth muscle of human uterus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human uterus (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1146, Anti-Calponin 1, clone 9D14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in smooth muscle of human uterus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human uterus (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1146, Anti-Calponin 1, clone 9D14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in smooth muscle of human uterus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and mouse colon (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1146, Anti-Calponin 1, clone 9D14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in smooth muscle of mouse colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and mouse colon (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1146, Anti-Calponin 1, clone 9D14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in smooth muscle of mouse colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and mouse colon (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1146, Anti-Calponin 1, clone 9D14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in smooth muscle of mouse colon tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of human skeletal muscle cells was performed using a 1:100 dilution of Cat. No. ZRB1146, Anti-Calponin 1, clone 9D14 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of human skeletal muscle cells was performed using a 1:100 dilution of Cat. No. ZRB1146, Anti-Calponin 1, clone 9D14 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of human skeletal muscle cells was performed using a 1:100 dilution of Cat. No. ZRB1146, Anti-Calponin 1, clone 9D14 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Human skeletal muscle tissue lysate was probed with Cat. No. ZRB1146, Anti-Calponin 1, clone 9D14 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Calponin-1 (~33 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 9D14, recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| mol wt | calculated mol wt 33.17 kDa |
| observed mol wt ~33 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable (peptide) | |
| UniProt accession no. | P51911  |
| Application: | Anti-Calponin 1, clone 9D14 ZooMAb, Cat. No. ZRB1146, is a recombinant rabbit monoclonal antibody that specifically targets Calponin 1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calponin 1 in human skeletal muscle. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Calponin 1 in human uterus and mouse colon tissue sections. Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Calponin-1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 9D14 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Calponin-1. It targets an epitope within 13 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Calponin-1 (UniProt: P51911; also known as Basic calponin, Calponin H1 smooth muscle) is encoded by the CNN1 gene (Gene ID:1264) in human. Calponin is an actin filament-associated regulatory protein that is expressed in smooth muscle and non-muscle cells of vertebrates. Three isoforms of Calponin have been reported that inhibit actin-activated myosin ATPase activity and stabilize the actin cytoskeleton. Calponin 1 is specifically expressed in smooth muscle cells is involved in fine-tuning of smooth muscle contractility. Calponin 2 is found in multiple cell types, including developing smooth muscles and Calponin 3 is found in the neurons, astrocytes and glial cells. Calponin 1 can bind to actin, calmodulin, troponin C, and tropomyosin. Calponin-1 (and other calponins) has a calponin homology (CH) domain at its N-terminal (aa 28-131) that can function either as a part of actin-binding site or serve as a regulatory structure for actin-binding activity. It also has three calponin-like domains (aa 164-189; 204-229; and 243-268) next to the C-terminal region that are conserved in all calponins. The C-terminal segment varies among different calponins and has differentiated effects on weakening the binding of calponin to F-actin. Deletion of the C-terminal tail segment is shown to enhance the actin-binding and bundling activities of calponins. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.(Ref.: Liu, R., and Jin, J-P (2016). Gene 585(1); 143-153). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |