Anti-p21 Antibody, clone 5G7, ZooMAb^® Rabbit Monoclonal

SIGMA/ZRB1141 - recombinant, expressed in HEK 293 cells

Synonym: Anti-CAP20; Anti-CDKN1; Anti-CIP1; Anti-MDA-6; Anti-P21; Anti-SDI1; Anti-WAF1; Anti-p21CIP1

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-ZRB1141-25UL	25 µL	$223.00 1/EA	Add To Favorites
45-ZRB1141-4X25UL	25 µL	$460.00 1/EA	Add To Favorites

Immunohistochemistry (Paraffin)
Enhanced Validation-Recombinant Antibody Technology
Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human cervical cancer (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1141, Anti-p21, clone 5G7 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in human cervical cancer tissue sections.

Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HUVEC was performed using a 1:100 dilution of Cat. No. ZRB1141, Anti-p21, clone 5G7 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.

Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH 3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1141, Anti-p21, clone 5G7 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.

Western Blotting Enhanced Validation-Recombinant Antibody Technology SW-480 cell lysate was probed with Cat. No. ZRB1141, Anti-p21, clone 5G7 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates p21 (~19 kDa).

12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.

12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.

12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.

Properties
Descriptions
Safety Info.
Basic Data

antibody form	purified antibody
antibody product type	primary antibodies
biological source	rabbit (recombinant)
clone	5G7, recombinant monoclonal
conjugate	unconjugated
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation
form	lyophilized
greener alternative category	Aligned
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry .
isotype	IgG
mol wt	calculated mol wt 18.12 kDa
	observed mol wt ~19 kDa
packaging	antibody small pack of 25 μL
product line	ZooMAb^® learn more
recombinant	expressed in HEK 293 cells
shipped in	ambient
species reactivity	mouse, human
storage temp.	2-8°C
technique(s)	immunocytochemistry: suitable
	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
	western blot: suitable (peptide)
UniProt accession no.	P38936

Application:	Anti-p21, clone 5G7 ZooMAb , Cat. No. ZRB1141, is a recombinant rabbit monoclonal antibody that specifically targets p21-Waf1-Cip1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Application:	Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p21 in HUVEC and NIH 3T3 cells. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected p21 in human cervical cancer tissue sections. Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
General description:	ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here.
Immunogen:	His-tagged full-length recombinant human Cyclin-dependent kinase inhibitor 1 (p21).
Legal Information:	ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form:	Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution:	300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Specificity:	Clone 5G7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects p21/Waf1/Cip1 protein.
Storage and Stability:	Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Target description:	Cyclin-dependent kinase inhibitor 1 (UniProt: P38936; also known as CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21) is encoded by the CDKN1A (also known as CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1) gene (Gene ID:1026) in human. p21 belongs to the Cip and Kip family of CDK inhibitors. It binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. It binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity. However, its effects on G2/M kinases is minimal. It binds the cyclin subunit through a conserved Cy1 motif in the N-terminal half and through a weaker and Cy2 motif in the C-terminal half. p21 also inhibits proliferating cell nuclear antigen (PCNA) and binds to and blocks the inactivation of retinoblastoma protein (Rb), which is essential for cell cycle progression. It is an important effector of p53 and is important in the DNA-damage checkpoint. It is expressed in all adult tissues, with 5-fold lower levels observed in the brain. Is cyclin binding site is localized to amino acids 17-24 and CDK binding occurs in the region of 53-58 residues. p21 is shown to suppress tumor growth by promoting cell cycle arrest in response to various stimuli. However, it is often misregulated in various human cancers. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Abbas, T., and Dutta, A (2009). Nat Rev Cancer. 9(6); 400-414).

WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352203

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