Anti-LAMP-1 Antibody, clone 2E9 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1131 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1131, Anti-LAMP-1, clone 2E9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/punctate cytoplasmic/membranous staining was observed in trophoblasts of human placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1131, Anti-LAMP-1, clone 2E9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/punctate cytoplasmic/membranous staining was observed in trophoblasts of human placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1131, Anti-LAMP-1, clone 2E9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/punctate cytoplasmic/membranous staining was observed in trophoblasts of human placenta tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human placenta tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1131, Anti-LAMP-1, clone 2E9 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human placenta tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1131, Anti-LAMP-1, clone 2E9 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human placenta tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1131, Anti-LAMP-1, clone 2E9 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from HeLa (Lane 1), K562 (Lane 2), Jurkat (Lane 3), and Cos-7 (Lane 4) cells were probed with Cat. No. ZRB1131, Anti-LAMP-1, clone 2E9 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates LAMP-1 (~110 kDa).
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million K562 cells was performed using 0.1 μg of Cat. No. ZRB1131, Anti-LAMP-1, clone 2E9 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit IgG secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
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Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2E9, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 44.88 kDa |
| observed mol wt ~110 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_005552.3  |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | monkey, human |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P11279 |
| Application: | Quality Control Testing Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LAMP-1 in HeLa cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LAMP-1 in K562, Jurkat, and Cos-7 cell lysates. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected LAMP-1 in human placenta tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected LAMP-1 in human placenta tissue sections. Flow Cytometry Analysis: 0.1 μg from a representative lot detected LAMP-1 in one million K562 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-LAMP-1, clone 2E9 ZooMAb®, Cat. No. ZRB1131, is a recombinant Rabbit monoclonal antibody that specifically detects LAMP-1 and is tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal, lumenal domain of human LAMP-1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2E9 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects LAMP-1. It targets an epitope within 14 amino acids from the N-terminal, lumenal domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Lysosome-associated membrane glycoprotein 1 (UniProt: P11279; also known as LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a) is encoded by the LAMP1 gene (Gene ID: 3916) in human. LAMP-1 and LAMP-2 are two highly glycosylated proteins found in the lysosomal membrane. They may contribute up to 50% of all proteins present in the lysosomal membranes. They are known to maintain the lysosomal acidification and lysosomal membrane integrity. Extensive N-glycosylation on LAMP-1 and 2 to protects them from the lysosomal proteolysis. However, higher levels of poly-N-acetyllactosamines on LAMP-1 and 2 have been correlated with decreased cellular differentiation and enhanced metastatic potential. Over-expression of LAMP-1 has also been implicated in breast cancer development and progression. LAMP-1 is a single-pass type I transmembrane protein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form that contains a large lumenal domain (aa 29-382), a transmembrane domain (aa 383-405), and a short C-terminal cytoplasmic tail (aa 406-417). LAMP-1 presents carbohydrate ligands to selectins and is also reported to act as a receptor for Lassa virus protein. It is reported to shuttle between lysosomes, endosomes, and the plasma membrane. Two isoforms of LAMP-1 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, Q., et al. (2017). Oncol. Lett. 14(4); 4729 - 4735; Tan, K-P., et al. (2016). Cell Death Dis. 7, e2347). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |