Anti-Synapsin-1 Antibody, clone 1G5 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1119 - recombinant, expressed in HEK 293 cells
Synonym: Brain protein 4.1; Synapsing I
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in granular and molecular layers, while moderate cytoplasmic staining was observed in Purkinje cells of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in granular and molecular layers, while moderate cytoplasmic staining was observed in Purkinje cells of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in granular and molecular layers, while moderate cytoplasmic staining was observed in Purkinje cells of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neuropil of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neuropil of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neuropil of human cerebral cortex tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of E18 rat cortical neurons was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). Target is found in golgi or synaptic vesicles of neurons so punctate cyto or just cyto staining is typically seen.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of E18 rat cortical neurons was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). Target is found in golgi or synaptic vesicles of neurons so punctate cyto or just cyto staining is typically seen.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of E18 rat cortical neurons was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). Target is found in golgi or synaptic vesicles of neurons so punctate cyto or just cyto staining is typically seen.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Rat cerebellum tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). Cytoplasmic/membranous staining was observed in granular and moleular layers of rat cerebellum tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Rat cerebellum tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). Cytoplasmic/membranous staining was observed in granular and moleular layers of rat cerebellum tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Rat cerebellum tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). Cytoplasmic/membranous staining was observed in granular and moleular layers of rat cerebellum tissue sections.
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Synapsin-1 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1119, Anti-Synapsin-1, clone 1G5 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Synapsin -1 peptide displayed a KD value of 8.2 x 10-8 whereas its binding to non-specific peptide displayed a KD of 1.3 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| biological source | rabbit |
| clone | recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 74.11 kDa |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, rat, human |
| species reactivity (predicted by homology) | monkey, canine |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| UniProt accession no. | P17600  |
| Application: | Quality Control Testing Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Synapsin-1 in rat cerebellum tissue sections.Tested applicationsImmunohistoch |
| Application: | Anti-Synapsin-1, clone 1G5 ZooMAb, Cat. No. ZRB1119, is a recombinant Rabbit monoclonal antibody that specifically detects Synapsin-1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Synapsin-1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1G5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Synapsin-1. It targets an epitope within 19 amino acids from the C-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Synapsin-1 (UniProt: P17600; also known as Brain protein 4.1, Synapsing I) is encoded by the SYN1 gene (Gene ID: 6853) in human. Synapsins are abundant phosphoproteins expressed primarily in the brain. Synapsins I and II are expressed at the highest levels and are specifically localized to presynaptic terminals, whereas synapsin III is expressed at very low levels and is also found in cell bodies and growth cones. Synapsins I and II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1-2 months of life. The N-terminal region of all synapsin have highly conserved domains A-C, while the domains D-J in the C-terminal region differ between isoforms. Domain A is shown to interact with phospholipids and this interaction is neutralized by phosphorylation of a serine by PKA and CaM Kinase I. Domain B connects domains A and C. Domain C displays most extensive homology across synapsins and mediates many of the core functions of synapsins. Synapsin I is a homodimeric protein that is present in all nerve terminals and is associated with synaptic vesicles, located at the cytoplasmic face of these vesicles. It is involved in the regulation of neurotransmitter release. In the resting state dephospho-synapsin I bind to the transmitter vesicle and reduces the availability of vesicle to fuse with presynaptic membrane. However, in the stimulated state, phosphorylated synapsin I can achieve a fusion-ready state. Synapsin I is shown to be a substrate for at least four different protein kinases and phosphorylation at serine 9 is reported to dissociate synapsin from synaptic vesicles. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Song, S-H., and Augustine GJ (2015). Mol. Cells. 30; 38(11); 936-940). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |