Anti-Aggrecan Antibody, clone 4B10 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1117 - recombinant, expressed in HEK 293 cells
Synonym: CSPCP; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cartilage (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1117, Anti-Aggrecan, clone 4B10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in chondrocytes of human cartilage tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cartilage (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1117, Anti-Aggrecan, clone 4B10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in chondrocytes of human cartilage tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cartilage (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1117, Anti-Aggrecan, clone 4B10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in chondrocytes of human cartilage tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of human chondrocytes was performed using a 1:100 dilution of Cat. No. ZRB1117, Anti-Aggrecan, clone 4B10 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). Is a protein expressed primarily (or exclusively) in cartilage.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of human chondrocytes was performed using a 1:100 dilution of Cat. No. ZRB1117, Anti-Aggrecan, clone 4B10 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). Is a protein expressed primarily (or exclusively) in cartilage.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of human chondrocytes was performed using a 1:100 dilution of Cat. No. ZRB1117, Anti-Aggrecan, clone 4B10 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). Is a protein expressed primarily (or exclusively) in cartilage.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Human Chondrocytes (Lane 1) and Human Mesenchymal Stem (Lane 2) Cells were probed with Cat. No. ZRB1117, Anti-Aggrecan, clone 4B10 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Aggrecan (~300 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Aggrecan peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1117, Anti-Aggrecan, clone 4B10 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Aggrecan peptide displayed a KD value of 2.4 x 10-8 whereas its binding to non-specific control peptide displayed a KD of 4.2 x 10-5.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| biological source | rabbit |
| clone | recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 261.33 kDa |
| observed mol wt ~300 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P16112  |
| Application: | Quality Control Testing Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Aggrecan in Human Chondrocytes.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Aggrecan in Human Mesenchymal Stem Cells.Affinity Binding Assay: A representative lot of this antibody bound Aggrecan peptide with a KD of 2.4 x 10-8 in an affinity binding assay.Immunohistochemistr |
| Application: | Anti-Aggrecan, clone 4B10 ZooMAb, Cat. No. ZRB1117, is a recombinant Rabbit monoclonal antibody that targets Aggrecan and has been tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Aggrecan. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4B10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Aggrecan. It targets an epitope within 21 amino acids from the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Aggrecan core protein (UniProt: P16112; also known as Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1) is encoded by the ACAN (also known as AGC1, CSPG1, MSK16) gene (Gene ID: 176) in human. Aggrecan, a secreted proteoglycan, is a major component of extracellular matrix of cartilaginous tissues. Its major function is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region and may play a regulatory role in the matrix assembly of the cartilage. It is synthesized with a signal peptide (aa 1-16), which is subsequently cleaved off to generate the mature aggrecan core protein. Aggrecan contains two globular domains, G1 and G2 that comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. The G1 region is located in the N-terminus of the core protein and interacts with hyaluronic acid (HA). G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B′ motifs. The G2 region is composed of two B-like domains, but does not have the ability to interact with HA. G2 region is separated from the G1 region by the interglobular domain, which is a prominent site for proteolysis. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3. Aggrecan is present in the extracellular matrix in the form of proteoglycan aggregates, in which many aggrecan molecules interact with hyaluronan and a link protein stabilizes each interaction. It is reported that aggrecan structure changes throughout the life cycle due to changes in synthetic and degradative events. Changes due to synthesis alter the structure of the chondroitin sulfate and keratan sulfate chains, whereas those due to degradation cause cleavage of all components of the aggregate. Aggrecan is also present in most of the retina including the outer nuclear layer, the outer plexiform layer, and the inner plexiform layer. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |