Anti-Glucagon Antibody, clone 6D6 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1115 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1115, Anti-Glucagon, clone 6D6 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. ECM staining was observed in islets of human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1115, Anti-Glucagon, clone 6D6 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. ECM staining was observed in islets of human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1115, Anti-Glucagon, clone 6D6 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. ECM staining was observed in islets of human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1115, Anti-Glucagon, clone 6D6 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. ECM staining was observed in islets of human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1115, Anti-Glucagon, clone 6D6 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. ECM staining was observed in islets of human pancreas tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human pancreas tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1115, Anti-Glucagon, clone 6D6 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the ECM.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human pancreas tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1115, Anti-Glucagon, clone 6D6 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the ECM.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human pancreas tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1115, Anti-Glucagon, clone 6D6 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the ECM.
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Glucagon peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1115, Anti-Glucagon, clone 6D6 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Glucagon peptide displayed a KD of 1.0 x 10-12, whereas binding to non-specific control peptide displayed a KD of 1.1 x 10-5.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 6D6, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 3.49 kDa |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_002045.1  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | rat, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| UniProt accession no. | P01275 |
| Application: | Quality Control Testing Evaluated by Immunohistochemistry (Paraffin) in human pancreas tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Glucagon in human pancreas tissue sections. Tested applications Affinity Binding Assay: A representative lot of this antibody bound Glucagon with a KD of 1.0 x 10-12 in an affinity binding assay. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Glucagon in human pancreas tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Glucagon, clone 6D6 ZooMAb®, Cat. No. ZRB1115, is a recombinant Rabbit monoclonal antibody that targets Glucagon and is tested for use in Affinity Binding Assay, Immunofluorescence, and Immunohistochemistry (Paraffin). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to full-length human glucagon. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 6D6 is a Rabbit recombinant monoclonal antibody that specifically detects glucagon. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Pro-glucagon (UniProt: P01275) is encoded by the GCG gene (Gene ID: 2641) in human. Pro-glucagon is a 180 amino acid peptide that is post-translationally processed in a tissue-specific manner in pancreatic alpha cells and intestinal L cells. In pancreatic alpha cells, glucagon (aa 53-81) is produced by cleavage of pro-glucagon by proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2/PC2). It is secreted in response to low levels of blood glucose. Hence, its circulating levels are low in the prandial state and increase 2 3 fold in response to fasting or hypoglycemia. Its actions are mediated via the glucagon receptor (GCGR), a G protein-coupled receptor that is most abundantly expressed in the liver. Traces of GCGR are also reported in several extrahepatic tissues, such as the brain, heart, kidney, gastrointestinal tract, and adipose tissues. It regulates blood glucose levels by increasing gluconeogenesis and reducing glycolysis. As a counterregulatory hormone of insulin, glucagon raises plasma glucose levels in response to insulin-induced hypoglycemia. In diabetic subjects, it plays a role in initiating and maintaining hyperglycemic condition. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |