Anti-Helicase MOV-10 Antibody, clone 3B8 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1112 - recombinant, expressed in HEK 293 cells
Synonym: Armitage homolog; Moloney leukemia virus 10 protein
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10 dilution of Cat. No. ZRB1112, Anti-Helicase MOV-10, clone 3B8 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic staining was primarily observed in pancreatic islets, with a lesser degree of staining visible in acinar cells of human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10 dilution of Cat. No. ZRB1112, Anti-Helicase MOV-10, clone 3B8 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic staining was primarily observed in pancreatic islets, with a lesser degree of staining visible in acinar cells of human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10 dilution of Cat. No. ZRB1112, Anti-Helicase MOV-10, clone 3B8 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic staining was primarily observed in pancreatic islets, with a lesser degree of staining visible in acinar cells of human pancreas tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZRB1112, Anti-Helicase MOV-10, clone 3B8 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZRB1112, Anti-Helicase MOV-10, clone 3B8 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZRB1112, Anti-Helicase MOV-10, clone 3B8 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from HepG2 (Lane 1) and Jurkat (Lane 2) cells were probed with Cat. No. ZRB1112, Anti-Helicase MOV-10, clone 3B8 ZooMAb® Rabbit Monoclonal (1:1,000 silution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Helicase MOV-10 (~114 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 μg/mL of of Helicase MOV-10 peptide (Run 1) or non-specific control peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1112, Anti-Helicase MOV-10, clone 3B8 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Helicase MOV-10 peptide displayed a KD value of 2.4 x 10-7 whereas its binding to non-specific peptide displayed a KD of 3.1 x 10-4.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 3B8, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 113.67 kDa |
| observed mol wt ~114 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| western blot: suitable |
| Application: | Anti-Helicase MOV-10, clone 3B8 ZooMAb, Cat. No. ZRB1112, is a recombinant Rabbit monoclonal antibody that specifically targets Helicase MOV-10 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blott |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Helicase MOV-10 in HepG2 cell lysate. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Helicase MOV-10 in HepG2 cells. Immunohistochemistry (Paraffin) Analysis: A 1:10 dilution from a representative lot detected Helicase MOV-10 in human pancreas tissue sections. Affinity Binding Assay:: A representative lot of this antibody bound Helicase MOV-10 peptide with a KD of 2.4 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Helicase MOV-10. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Helicase MOV-10. It targets an epitope within 20 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Helicase MOV-10 (UniProt: EC:3.6.4.13; also known as Armitage homolog, Moloney leukemia virus 10 protein) is encoded by the MOV10 (also known as KIAA1631) gene (Gene ID: 4343) in human. MOV-10 is an evolutionarily conserved member of the UPF1p family of SF-1 ATP-dependent RNA helicases that serves as a putative 5′ to 3′ RNA helicase and contributes to UPF1 mRNA target degradation by translocation along 3′ UTRs. It is shown to inhibit retrovirus replication and overexpression of MOV-10 is shown to severely impair infectivity of HIV-1 and other lentiviruses. It is required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). MOV-10 is also shown to be interferon-inducible RNA helicase and can exhibit antiviral activity independent of its helicase function, against several positive- and negative-strand RNA viruses by enhancing type -I interferon induction. MOV-10 is reported to severely restrict human LINE1 (L1), Alu, and SVA retrotransposons. It associates with the L1 ribonucleoprotein particle, along with other RNA helicases including DDX5, DHX9, DDX17, DDX21, and DDX39A. MOV-10 is co-enriched in cytoplasmic foci along with TUT4. In developing neurons, it is shown to localize both in nucleus and cytoplasm, however, in adults it is found only in the cytoplasmic. It is also required for embryonic viability and for normal central nervous system development and function. It is reported to play two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis and regulates cytoskeletal mRNAs to influence neurite outgrowth. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goodier, JL., et al. (2012). PLoS Genet 8(10); e1002941; Cuevas RA., et al. (2016). J. Immunol. 196(9); 3877-3886). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |