Anti-Brn3a Antibody, clone 4L5, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1098 - recombinant, expressed in HEK 293 cells
Synonym: Anti-Anti-ATITHS; Anti-Anti-BRN3A; Anti-Anti-Oct-T1; Anti-Anti-RDC-1; Anti-Anti-brn-3A
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control human retina (A) human retina (Middle image), and mouse retina (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10,000 dilution of Cat. No. ZRB1098, Anti-Brn3a, clone 4L5 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. In human retina, nuclear staining was observed in subsets of cells within GCL & ONL, while membranous staining was observed in areas extending from IPL, INL & OPL (Middle image). In mouse retina, nuclear staining was observed mostly in INL, with a lesser degree of staining in GCL (B).
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control human retina (A) human retina (Middle image), and mouse retina (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10,000 dilution of Cat. No. ZRB1098, Anti-Brn3a, clone 4L5 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. In human retina, nuclear staining was observed in subsets of cells within GCL & ONL, while membranous staining was observed in areas extending from IPL, INL & OPL (Middle image). In mouse retina, nuclear staining was observed mostly in INL, with a lesser degree of staining in GCL (B).
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control human retina (A) human retina (Middle image), and mouse retina (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10,000 dilution of Cat. No. ZRB1098, Anti-Brn3a, clone 4L5 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. In human retina, nuclear staining was observed in subsets of cells within GCL & ONL, while membranous staining was observed in areas extending from IPL, INL & OPL (Middle image). In mouse retina, nuclear staining was observed mostly in INL, with a lesser degree of staining in GCL (B).
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control human retina (A) human retina (Middle image), and mouse retina (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10,000 dilution of Cat. No. ZRB1098, Anti-Brn3a, clone 4L5 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. In human retina, nuclear staining was observed in subsets of cells within GCL & ONL, while membranous staining was observed in areas extending from IPL, INL & OPL (Middle image). In mouse retina, nuclear staining was observed mostly in INL, with a lesser degree of staining in GCL (B).
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Neuro2A cell line were performed using a 1:40 dilution of Cat. No. ZRB1098, Anti-Brn3a, clone 4L5 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Neuro2A cell line were performed using a 1:40 dilution of Cat. No. ZRB1098, Anti-Brn3a, clone 4L5 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Neuro2A cell line were performed using a 1:40 dilution of Cat. No. ZRB1098, Anti-Brn3a, clone 4L5 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Human (Lane 1), mouse (Lane 2), and rat (Lane 3) brain tissue lysates were probed with Cat. No. ZRB1098, Anti-Brn3a, clone 4L5 ZooMAb® Rabbit Monoclonal (1:1,000 (Lane 1) and 1:10,000 (Lane 2 and 2)). Proteins were visualized using a Donkey Anti-Rabbit IgG + secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Brn3a (~48 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 4L5, recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 42.70 kDa |
| observed mol wt ~48 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, human, mouse |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: suitable (peptide) | |
| UniProt accession no. | Q01851  |
| Application: | Anti-Brn3a, clone 4L5 ZooMAb , Cat. No. ZRB1098, is a highly specific rabbit recombinant monoclonal antibody antibody that targets Brn-3a and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: 1:1,000-1:10,000 from a representative lot detected Brn3a in mouse and rat brain tissue lysates Immunocytochemistry Analysis: A 1:40 dilution from a representative lot detected Brn3a in Neuro2A cell line. Immunohistochemistry (Paraffin) Analysis: A 1:10,000 dilution from a representative lot detected Brn3a in human and mouse retina tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugted linear peptide corresponding to 19 amino acids from the internal region of human homeobox/POU domain protein 3A (Brn-3a). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4L5 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects Brain-specific homeobox/POU domain protein 3A, (Brn-3a). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | POU domain, class 4, transcription factor 1 (UniProt: Q01851; also known as Brain-specific homeobox/POU domain protein 3A, Brain-3A, Brn-3A, Homeobox/POU domain protein RDC-1, Oct-T1) is encoded by the POU4F1 (also known as BRN3A, RDC1) gene (Gene ID: 5457) in human. Brn-3A is a multifunctional transcription factor with different regions mediating its different effects. It regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It is expressed in the brain and the retina and is present in the developing brain, spinal cord and eye. Its expression peaks early in embryogenesis and is undetectable 14 days after birth. Its C-terminal domain can act as both DNA-binding domain and a transcriptional activator. The N-terminal domain is also required for transactivation activity on some target genes acting as a discrete activation domain. Brn-3A acts by binding to sequences related to the consensus octamer motif 5′-ATGCAAAT-3′ in the regulatory regions of its target genes. The POU-specific domain of Brn-3A is localized to amino acids 261-338. Brn-3A is shown to activate Bcl-2 expression and protect neuronal cells from apoptosis. Two isoforms of Brn-3A have been described that are produced by alternative splicing. Isoform 2 can act as a transcription factor but cannot regulate the expression of the same subset of genes and does not have any anti-apoptotic effect on neuronal cells. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |