Anti-Green Fluorescent Protein Antibody, clone 4I10, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1097 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of hGFAP-GFP transgenic mouse brain tissue sections was performed using a 1:250 dilution of Cat. No. ZRB1097, Anti-Green Fluorescent Protein, clone 4I10 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody transgenic mouse brain tissue showing GFP fluorescence (Green). Actin filaments have been labeled with Alexa Fluor® 594 dye (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm subsets of neurons of hGFAP-GFP mouse brain cortex.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of hGFAP-GFP transgenic mouse brain tissue sections was performed using a 1:250 dilution of Cat. No. ZRB1097, Anti-Green Fluorescent Protein, clone 4I10 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody transgenic mouse brain tissue showing GFP fluorescence (Green). Actin filaments have been labeled with Alexa Fluor® 594 dye (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm subsets of neurons of hGFAP-GFP mouse brain cortex.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of hGFAP-GFP transgenic mouse brain tissue sections was performed using a 1:250 dilution of Cat. No. ZRB1097, Anti-Green Fluorescent Protein, clone 4I10 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody transgenic mouse brain tissue showing GFP fluorescence (Green). Actin filaments have been labeled with Alexa Fluor® 594 dye (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm subsets of neurons of hGFAP-GFP mouse brain cortex.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of hGFAP-GFP transgenic mouse brain tissue sections was performed using a 1:250 dilution of Cat. No. ZRB1097, Anti-Green Fluorescent Protein, clone 4I10 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody transgenic mouse brain tissue showing GFP fluorescence (Green). Actin filaments have been labeled with Alexa Fluor® 594 dye (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm subsets of neurons of hGFAP-GFP mouse brain cortex.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HEK 293T cells transfected with GFP transfected membrane protein was performed using a 1:1,000 dilution of Cat. No. ZRB1097, Anti-Green Fluorescent Protein, clone 4I10 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody conjugated to Cy3 (Red). Anti-GFP. Green, autofluorescence GFP (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains GFP tagged cell membrane protein and cytoplasm of transfected cells.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HEK 293T cells transfected with GFP transfected membrane protein was performed using a 1:1,000 dilution of Cat. No. ZRB1097, Anti-Green Fluorescent Protein, clone 4I10 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody conjugated to Cy3 (Red). Anti-GFP. Green, autofluorescence GFP (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains GFP tagged cell membrane protein and cytoplasm of transfected cells.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HEK 293T cells transfected with GFP transfected membrane protein was performed using a 1:1,000 dilution of Cat. No. ZRB1097, Anti-Green Fluorescent Protein, clone 4I10 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody conjugated to Cy3 (Red). Anti-GFP. Green, autofluorescence GFP (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains GFP tagged cell membrane protein and cytoplasm of transfected cells.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HEK 293T cells transfected with GFP transfected membrane protein was performed using a 1:1,000 dilution of Cat. No. ZRB1097, Anti-Green Fluorescent Protein, clone 4I10 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody conjugated to Cy3 (Red). Anti-GFP. Green, autofluorescence GFP (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains GFP tagged cell membrane protein and cytoplasm of transfected cells.
Western Blotting Enhanced Validation-Recombinant Antibody Technology GFP spiked into a HeLa cell lysate was probed with Cat. No. ZRB1097, Anti-Green Fluorescent Protein, clone 4I10 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Green Fluorescent Protein (~30 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 4I10, recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 26.89 kDa |
| observed mol wt ~30 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | all |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| western blot: suitable (peptide) | |
| UniProt accession no. | P42212  |
| Application: | Anti-Green Fluorescent Protein, clone 4I10 ZooMAb , Cat. No. ZRB1097, is a highly specific recombinant rabbit monoclonal antibody that targets Green Fluorescent Protein and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting. |
| Application: | Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Green Fluorescent Protein in GFP spiked into a HeLa cell lysate. Immunofluorescence Analysis: A 1:250 dilution from a representative lot detected Green Fluorescent Protein in hGFAP-GFP transgenic mouse brain tissue sections. Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Green Fluorescent Protein in membranes of HEK 293T cells transfected with GFP. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | His-tagged full-length recombinant Green fluorescent protein (GFP) from Aequorea victoria (Jelly fish). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4I10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Green fluorescent protein (GFP) from Aequorea victoria (Jelly Fish). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Green fluorescent protein (UniProt: P42212; also known as GFP) is encoded by the GFP gene in Aequorea victoria (Jelly Fish). GFP is a 27 kDa protein from jellyfish that serves as an energy-transfer acceptor. It transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer and it fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. GFP has an absorption max at 395 nm and the emission peaks at 507 to 510 nm. However, the exact value of emission max depends on the environment of the chromophore. GFP is an important tool in cell biology research that displays stability under fixation conditions and is suitable for a variety of applications, such as tracing cell lineage tracer, reporter of gene expression, or exploring protein-protein interactions. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |