Anti-EZH2 Antibody, clone 6I10 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1095 - recombinant, expressed in HEK 293 cells
Synonym: Histone-lysine N-methyltransferase EZH2
Product Type: Chemical
antibody form | purified antibody |
antibody product type | primary antibodies |
biological source | rabbit |
clone | 6I10, recombinant monoclonal |
conjugate | unconjugated |
description | recombinant, expressed in HEK 293 cells |
enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
epitope sequence | Internal |
form | lyophilized |
greener alternative category | Aligned |
greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
isotype | IgG |
mol wt | calculated mol wt 85.36 kDa |
observed mol wt ~85 kDa | |
packaging | antibody small pack of 25 μL |
product line | ZooMAb® learn more |
Protein ID accession no. | NP_001190176.1 |
purified by | using Protein A |
Quality Level | 200 |
recombinant | expressed in HEK 293 cells |
shipped in | ambient |
species reactivity | mouse, human, rat |
species reactivity (predicted by homology) | horse, bovine, monkey, canine |
storage temp. | 2-8°C |
technique(s) | affinity binding assay: suitable |
immunocytochemistry: suitable | |
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
western blot: suitable | |
UniProt accession no. | Q15910 |
Application: | Quality Control Testing Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EZH2 in HeLa cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EZH2 in C6, MDA-MB-231, and NIH3T3 cell lysates. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected EZH2 in human testis tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected EZH2 in HeLa, HepG2, and NIH 3T3 cells. Affinity Binding Assay: A representative lot of this antibody bound EZH2 with a KD of 5.7 x 10-8 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
Immunogen: | KLH-conjugated linear peptide corresponding to 18 amino acids from the internal region of human Histone-lysine N-methyltransferase EZH2. |
Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
Specificity: | Clone 6I10 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Histone-lysine N-methyltransferase EZH2. It targets an epitope within 18 amino acids from the internal region. |
Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
Target description: | Histone-lysine N-methyltransferase EZH2 (UniProt: Q15910; also known as EC:2.1.1.356, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, EZH2) is encoded by the EZH2 (also known as KMT6) gene (Gene ID: 2146) in human. EZH2 is a polycomb group protein that is expressed in many tissues. It is the catalytic subunit of the PRC2/EED-EZH2 complex that methylates lysine 9 (H3K9me) and lysine 27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. It is shown to mono-, di- and trimethylate lysine 27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. It displays a preference for substrates with less methylation and loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 |
Storage Temp. | 2-8°C |
UNSPSC | 12352203 |