Anti-Arginase-1 Antibody, clone 3B17 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1088 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human liver (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1088, Anti-Arginase-1, clone 3B17 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in subsets of hepatocytes of human liver tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human liver (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1088, Anti-Arginase-1, clone 3B17 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in subsets of hepatocytes of human liver tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human liver (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1088, Anti-Arginase-1, clone 3B17 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in subsets of hepatocytes of human liver tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Human Liver tissue lysate was probed with Cat. No. ZRB1088, Anti-Arginase-1, clone 3B17 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Arginase-1 (~38 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Arginase-1 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1088, Anti-Arginase-1, clone 3B17 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Arginase-1 peptide displayed a KD of 1.3 x 10-7, whereas binding to non-specific control peptide displayed a KD of 5.3 x 10-3.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million HepG2 cells was performed using 0.1 μg of Cat. No. ZRB1088, Anti-Arginase-1, clone 3B17 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit IgG secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 3B17, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt ~34.74 kDa |
| observed mol wt ~38 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P05089  |
| Application: | Quality Control Testing Evaluated by Western Blotting in Human Liver tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Arginase-1 in Human Liver tissue lysate. Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Arginase-1in human liver tissue sections. Flow Cytometry Analysis: 0.1 μg from a representative lot detected Arginase-1 in one million HepG2 cells. Affinity Binding Assay; A representative lot of this antibody bound Arginase-1 peptides with a KD of 1.3 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Arginase-1, clone 3B17 ZooMAb®, Cat. No. ZRB1088, is a recombinant Rabbit monoclonal antibody that targets Arginase-1 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Arginase-1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3B17 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Arginase-1. It targets an epitope within 20 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Arginase-1 (UniProt: P05089; also known as EC:3.5.3.1, Liver-type arginase, Type I arginase) is encoded by the ARG1 gene (Gene ID: 383) in human. Arginase-1 is a manganese-containing liver enzyme that participates in urea cycle and converts L-arginine to urea and L-ornithine. It also functions to maintain L-arginine homeostasis in non-hepatic tissues where nitric oxide synthase (NOS) and arginase compete for the available intracellular arginine. Arginase-1 is also Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death, it is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. Although expression of arginase-1 is commonly associated with inflammation, fibrosis, and wound healing involving increased L-proline, polyamine, and Th2 cytokine production, macrophage-specific arginase-1 is reported to act as an inhibitor of inflammation and fibrosis following infection with the Th2-inducing pathogens. In murine species, arginase is induced in myeloid cells mainly by Th2 cytokines and inflammatory agents and participates in a variety of inflammatory diseases by down-regulation of nitric oxide synthesis, induction of fibrosis and tissue regeneration. Mutations in ARG1 gene are associated with Aargininemia that is characterized by elevated levels of arginine in the blood and cerebrospinal fluid, developmental delays, seizures, and mental retardation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Munder, M. (2009). Br. J. Pharmacol. 158(3); 638 651; Pesce, JT., et al. (2009). PLOS Pathogens 5(4); e1000371). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |