Anti-GABA Transporter-3 Antibody, clone 4E14 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1070 - recombinant, expressed in HEK 293 cells
Synonym: GAT-3; Sodium- and chloride-dependent GABA transporter 3; Solute carrier family 6 member 11
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1070, Anti-GABA Transporter-3, clone 4E14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in granular layer of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1070, Anti-GABA Transporter-3, clone 4E14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in granular layer of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1070, Anti-GABA Transporter-3, clone 4E14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in granular layer of rat cerebellum tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB1070, Anti-GABA Transporter-3, clone 4E14 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane (cytoplasm).
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB1070, Anti-GABA Transporter-3, clone 4E14 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane (cytoplasm).
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB1070, Anti-GABA Transporter-3, clone 4E14 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane (cytoplasm).
Western Blotting Enhanced Validation-Recombinant Antibody Technology Rat Brain tissue lysate was probed with Cat. No. ZRB1070, Anti-GABA Transporter-3, clone 4E14 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates GABA Transporter-3 (~58 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 μg/mL of GABA Transporter-3 peptide (Run 1) and non-specific control peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1070, Anti-GABA Transporter-3, clone 4E14 ZooMAb® Rabbit Monoclonal. The binding of this antibody to GABA Transporter-3 peptide displayed a KD value of 2.4 x 10-8 whereas its binding to non-specific control peptide displayed a KD of 1.3 x 10-4.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 4E14, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 69.95 kDa |
| observed mol wt ~58 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, rat |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P31647  |
| Application: | Anti-GABA Transporter-3, clone 4E14 ZooMAb, Cat. No. ZRB1070, is a recombinant Rabbit monoclonal antibody that detects GABA transporter 3 and is tested for use in is used in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GABA Transporter-3 in rat brain tissue lysate. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GABA Transporter-3 in PC12 cells. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GABA Transporter-3 in rat brain tissue sections. Affinity Binding Assay: A representative lot of this antibody bound GABA Transporter-3 peptide with a KD of 2.4 x 10-8 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of rat GABA Transporter 3. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4E14 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects GABA Transporter 3. It targets an epitope within 21 amino acids from the C-terminal, cytoplasmic domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Sodium- and chloride-dependent GABA transporter 3 (UniProt: P31647; also known as GAT-3, Solute carrier family 6 member 11, GABA Transporter 3) is encoded by the Slc6a11 (also known as Gabt3, Gat-3, Gat-b) gene (Gene ID: 79213) in rat. GABA is reported to be the main inhibitory neurotransmitter in the mammalian brain, particularly in the basal ganglia network. Following its release from the presynaptic terminal, it is rapidly cleared from the extracellular space by GABA transporters (GATs) resulting it s the termination of inhibitory synaptic transmission. Thus far, four different GATs have been described in literature, GAT-1, GAT-2, GAT-3, and the Betain/GABA transporter type 1. GAT-3 is a multi-pass membrane protein that has one extracellular domain (aa 147-220), two cytoplasmic domains (aa 1-53 and 567-627), and twelve helical domains. It is shown to be highly expressed in the glomerular layer of the olfactory bulb, the inner nucleus of the retina, the thalamic paraventricular nucleus, and the globus pallidus. It terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. The GABA-transporting action of GATs is dependent on the Na+ gradient across the membrane. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jin XT., et al. (2011). Front. Syst. Neurosci. 5; 63). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |