Anti-Treslin Antibody, clone 5L19 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1063 - recombinant, expressed in HEK 293 cells
Synonym: TopBP1-interacting checkpoint and replication regulator; TopBP1-interacting replication-
Product Type: Chemical
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH 3T3 cells was performed using a 1:100 dilution of Cat. No. ZRB1063, Anti-Treslin, clone 5L19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH 3T3 cells was performed using a 1:100 dilution of Cat. No. ZRB1063, Anti-Treslin, clone 5L19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH 3T3 cells was performed using a 1:100 dilution of Cat. No. ZRB1063, Anti-Treslin, clone 5L19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation-Recombinant Antibody Technology NIH3T3 cell lysate was probed with Cat. No. ZRB1063, Anti-Treslin, clone 5L19 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Treslin (~260 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 μg/mL of Treslin peptide (Run 1) and control peptides (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1063, Anti-Treslin, clone 5L19 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Treslin peptide displayed a KD value of 4.3 x 10-6 whereas its binding to control peptide displayed a KD of 1.9 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 5L19, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 210.86 kDa |
| observed mol wt ~260 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse |
| species reactivity (predicted by homology) | monkey, human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q7Z2Z1  |
| Application: | Anti-Treslin, clone 5L19 ZooMAb, Cat. No. ZRB1063, is a recombinant Rabbit monoclonal antibody that specifically targets Treslin and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting. |
| Application: | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Treslin in NIH 3T3 cells. Affinity Binding Assay: A representative lot of this antibody bound Treslin peptides with a KD of 4.3 x 10-6 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal region of human Treslin. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 5L19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Treslin. It targets an epitope within 25 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Treslin (UniProt: Q7Z2Z1; also known as TopBP1-interacting checkpoint and replication regulator, TopBP1-interacting, replication-stimulating protein) is encoded by the TICRR (also known as C15orf42) gene (Gene ID: 90381) in human. Treslin is a well conserved nuclear protein that associates with replicating chromatin. It serves as a regulator of DNA replication by promoting integration of Cdc45 into the replicative helicase. It is a key positive-regulatory target of cell-cycle control mechanisms and activation of Treslin by cyclin-dependent kinase (CDK) is shown to be essential for the initiation of replication. It possesses an unusually large number of potential CDK phosphorylation sites. Checkpoint-regulatory kinase Chk1 is shown to associate with a C-terminal domain of Treslin (TRCT) and mutations in the TRCT domain are known to abolish binding of Chk1 to Treslin and thereby eliminate its phosphorylation by Chk1. Disruption of the Treslin-Chk1 interaction is shown to increase initiation of chromosomal DNA replication. Two isoforms of Treslin have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kumagai, A., et al. (2010). Cell. 140(3); 349-359; Guo, C., et al. (2015). Mol. Cell. 57(3); 492-505). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |