Anti-ZFAND1 Antibody, clone 4K16 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1062 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Western Blotting Enhanced Validation - Recombinant Antibody Technology Nuclear extracts from NIH3T3 (Lane 1) and HeLa (Lane 2) cells were probed with Cat. No. ZRB1062, Anti-ZFAND1, clone 4K16 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ZFAND1 (~30 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 10 mg/mL of non-specific GST protein (Run 1) or recombinant ZFAND1 protein fragment (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1062, Anti-ZFAND1, clone 4K16 ZooMAb® Rabbit Monoclonal. The binding of this antibody to ZFAND1 protein displayed a KD of 6.4 x 10-7, whereas binding to non-specific GST protein displayed a KD of 1.9 x 10-3.
ELISA Enhanced Validation - Recombinant Antibody Technology 0.1 μg of ZFAND1 and GST control proteins were analyzed by ELISA using various dilutions (starting at 3 mg/mL) of Cat. No. ZRB1062, Anti-ZFAND1, clone 4K16 ZooMAb® Rabbit Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Rabbit secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 4K16, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 30.79 kDa |
| observed mol wt ~30 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_078975.2  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q8TCF1 |
| Application: | Quality Control Testing Evaluated by Western Blotting in HeLa cell nuclear extract. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZFAND1 in HeLa cell nuclear extract. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZFAND1 in NIH3T3 cell nuclear extract. ELISA Analysis: 300 μg/mL of this antibody detected ZFAND1 in an ELISA. Affinity Binding Assay: A representative lot of this antibody bound ZFAND1 with a KD of 6.4 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-ZFAND1, clone 4K16 ZooMAb®, Cat. No. ZRB1062, is a recombinant Rabbit monoclonal antibody that detects Zinc finger AN1-type-containing protein 1 (ZFAND1). (ZFAND1) and is tested for use in Affinity Binding Assay, ELISA, and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | His-tagged full-length recombinant human Zinc finger AN1-type-containing protein 1 (ZFAND1). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4K16 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects human Zinc finger AN1-type-containing protein 1 (ZFAND1). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | AN1-type zinc finger protein 1 (UniProt: Q8TCF1; also known as Zinc finger AN1-type-containing protein 1, ZFAND1) is encoded by the ZFAND1 gene (Gene ID: 79752) in human. ZFAND1 is a 268 amino acid protein with two ANI-type zinc fingers (aa 7-58 and 61-110) and a UBL domain. It plays a role in the regulation of cytoplasmic stress granules (SGs) turnover. It contains six conserved cysteines, two histidines, and has a zinc-bound /β fold. There are four isoforms of ZFAND1 have been described that are produced by alternative splicing. ZFAND1 is reported to interact with two key factors of protein degradation, the 26S proteasome and the ubiquitin-selective segregase p97, via its zinc finger and UBL domains, and recruits them to arsenite-induced stress granules. In the absence of ZFAND1, stress granules lack the 26S proteasome and p97 and accumulate defective ribosomal products. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Turakhiya, A., et al. (2018). Mol. Cell. 70(5); 906-919). |
| Storage Temp. | 2-8°C |