Anti-PHPT1 Antibody, clone 1D19 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1056 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Western Blotting Enhanced Validation - Recombinant Antibody Technology HepG2 (Lane 1) and A549 (Lane 2) cell lysates were probed with Cat. No. ZRB1056, Anti-PHPT1, clone 1D19 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PHPT1 (~14 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 10 mg/mL of recombinant PHPT1 protein (Run1) or non-specific GST protein (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1056, Anti-PHPT1, clone 1D19 ZooMAb® Rabbit Monoclonal. The binding of this antibody to PHPT1 protein displayed a KD of 1.0 x 10-12 whereas binding to non-specific GST protein displayed a KD of 1.2 x 10-3.
ELISA Enhanced Validation - Recombinant Antibody Technology 0.1 mg of PHPT1 protein (Blue) and GST protein (Orange) were analyzed by ELISA using three-fold dilutions (from 3,000 ng/mL to 0.46 ng/mL) of Cat. No. ZRB1056, Anti-PHPT1, clone 1D19 ZooMAb® Rabbit Monoclonal .Alkaline phosphatase-conjugated Goat Anti-Rabbit secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1D19, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 13.83 kDa |
| observed mol wt ~14 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_054891.2  |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9NRX4 |
| Application: | Quality Control Testing Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PHPT1 in HepG2 cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PHPT1 in A549 cell lysate. ELISA Analysis: 300 μg/mL from a representative lot detected PHPT1 protein in an indirect ELISA. Affinity Binding Assay:: A representative lot of this antibody bound PHPT1 protein with a KD of 1.0 x 10-12 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-PHPT1, clone 1D19 ZooMAb®, Cat. No. ZRB1056, is recombinant Rabbit monoclonal antibody that detects phosphohistidine phosphatase 1 (PHPT1) and is tested for use in Affinity Binding Assay, ELISA, and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | His-tagged full-length recombinant human Phosphohistidine phosphatase 1 (PHPT1). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1D19 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Phosphohistidine phosphatase 1 (PHTP1). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | 14 kDa phosphohistidine phosphatase (UniProt: Q9NRX4; also known as EC:3.9.1.3, Phosphohistidine phosphatase 1, PHPT1, Protein histidine phosphatase, PHP, Protein janus-A homolog) is encoded by the PHPT1 (also known as PHP14, CGI-202, HSPC141) gene (Gene ID: 29085) in human. Protein histidine phosphorylation is reported to exist widely in vertebrates and PHPT is the only phosphohistidine phosphatase described in mammals. It regulates phosphohistidine levels of several proteins including those involved in signaling, lipid metabolism, and potassium ion transport. It is expressed abundantly in heart and skeletal muscle and is also found in several other tissues. Human PHPT1 is composed of six β-strands that are flanked by two -helices. The substrate-binding site is shown to be positioned between two loops, one between 1 and β4, and the other between β5 and 2. This site contains multiple residues that are shown to be important for substrate binding and include Glu51, Tyr52, His53, Tyr93, and Met95. The active site His53, located at the beginning of helix 1, is identified as the catalytic residue and the NH groups of His53, Ala54, and Ala86 and the OH group of Ser94 act as anchors of substrate and stabilize phosphate binding to the active site. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Martin, DR., et al. (2016). Sci. Rep. 6; Article 23658; Gong, W. B. et al. Biochem. J. 418(2); 337-344; Busam, R. D. et al. (2006). J. Biol. Chem. 281(45); 33830-33834). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |