Anti-Somatostatin-28 Antibody, clone 2B19 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1042 - recombinant, expressed in HEK 293 cells
Synonym: Growth hormone release-inhibiting factor; Somatostatin
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1042, Anti-Somatostatin-28, clone 2B19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Extracellular staining was restricted to subsets of islet cells of human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1042, Anti-Somatostatin-28, clone 2B19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Extracellular staining was restricted to subsets of islet cells of human pancreas tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1042, Anti-Somatostatin-28, clone 2B19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Extracellular staining was restricted to subsets of islet cells of human pancreas tissue sections.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of human pancreas tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1042, Anti-Somatostatin-28, clone 2B19 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of human pancreas tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1042, Anti-Somatostatin-28, clone 2B19 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of human pancreas tissue sections was performed using a 1:100 dilution of Cat. No. ZRB1042, Anti-Somatostatin-28, clone 2B19 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 μg/mL of non-specific control peptide (Run 2) or human Somatostatin-28 peptide (Run 1) or non-specific control peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1042, Anti-Somatostatin-28, clone 2B19 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Somatostatin-28 peptide displayed a KD value of 1.1 x 10-7 whereas its binding to non-specific control peptide displayed a KD of 1.4 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2B19, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 12.73 kDa (for Somatostatin) |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | rat, sheep, bovine, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| UniProt accession no. | P61278  |
| Application: | Anti-Somatostatin-28, clone 2B19 ZooMAb, Cat. No. ZRB1042, is a recombinant Rabbit monoclonal antibody that targets Somatostatin-28 and is tested for use in Affinity Binding Assay, Immunofluorescence, and Immunohistochemistry (Paraffin). |
| Application: | Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Somatostatin-28 in human pancreas tissue sections. Affinity Binding Assay: A representative lot of this antibody bound human Somatostatin-28 peptide with a KD of 1.1 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 17 amino acids from human Somatistatin-28 (C-terminal region of somatostatin). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 8G9.2 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects human Somatstatin-28. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Somatostatin (UniProt: P61278; also known as Growth hormone release-inhibiting factor) is encoded by the SST gene (Gene ID: 6750) in human. Somatostatin, a tetradecapeptide, displays highest expression in the islets of Langerhans and in the brain. It is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off and the remaining chain can cleave into three chains: Neuronostatin (aa 31-43), Somatostatin-28 (aa 89-116), and Somatostatin-14 (aa 103-116). Somatostatin-28 is mainly produced by the intestinal enteroendocrine cells and somatostatin-14 is the predominant form present in the brain. Neuronostatin is believed to enhance low-glucose-induced glucagon release by pancreatic alpha cells. Somatostatin is shown to Inhibit the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. In the intestine it exerts inhibitory effects on various gastrointestinal functions, including gastric acid secretion, gastric emptying, intestinal motility, and release of various gastrointestinal hormones. Somatostatin actions are mediated through five distinct receptor subtypes known as SST1-5 that are G-protein coupled receptors. SST2 and 5 are shown to be the main mediators of the gastrointestinal effects of somatostatin. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |