Anti-PVR (CD155) Antibody, clone 4B3 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1036 - recombinant, expressed in HEK 293 cells
Synonym: CD155; NECL-5; Nectin-like protein 5; Poliovirus receptor
Product Type: Chemical
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of A431 cells was performed using a 1:100 dilution of Cat. No. ZRB1036, Anti-PVR (CD155), clone 4B3 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of A431 cells was performed using a 1:100 dilution of Cat. No. ZRB1036, Anti-PVR (CD155), clone 4B3 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of A431 cells was performed using a 1:100 dilution of Cat. No. ZRB1036, Anti-PVR (CD155), clone 4B3 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from HUVEC (Lane 1) and U251 cells (Lane 2) were probed with Cat. No. ZRB1036, Anti-PVR (CD155), clone 4B3 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PVR (CD155) (~65 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 μg/mL of PVR peptide (Run 1) or non-specific control peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1036, Anti-PVR (CD155), clone 4B3 ZooMAb® Rabbit Monoclonal. The binding of this antibody to PVR peptide displayed a KD value of 1.0 x 10-12 whereas its binding to non-specific peptide displayed a KD of 1.8 x 105.
Flow Cytometry Enhanced Validation-Recombinant Antibody Technology Staining of one million U937 was performed using 0.1 μg of Cat. No. ZRB1036, Anti-PVR (CD155), clone 4B3 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit IgG secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 4B3, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 45.3 kDa |
| calculated mol wt ~65 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P15151  |
| Application: | Anti-PVR (CD155), clone 4B3 ZooMAb, Cat. No. ZRB1036, is a recombinant Rabbit monoclonal antibody that targets PVR (CD155/Poliovirus receptor) and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PVR (CD155) in HUVEC lysate. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PVR (CD155) in A431 cells. Affinity Binding Assay: A representative lot of this antibody bound PVR (CD155) peptide with a KD of 1.0 x 10-12 in an affinity binding assay. Flow Cytometry Analysis: 0.1 μg from a representative lot detected PVR (CD155) in U937 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human poliovirus receptor. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4B3 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human poliovirus receptor. It targets an epitope within 16 amino acids from the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Poliovirus receptor (UniProt: P15151; also known as Nectin-like protein 5, NECL-5, CD155) is encoded by the PVR (also known as PVS) gene (Gene ID: 5817) in human. CD155 is a single-pass type I, N-glycosylated, membrane protein that mediates NK cell adhesion and triggers NK cell effector functions. It is ubiquitously expressed in various tissues and many human tumors are reported to significantly upregulate its expression. It binds two different NK cell receptors: CD96 and CD226 and these interactions accumulate at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and the target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. CD155 serves as a receptor for poliovirus and may play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with dynein light chain Tctex-type 1 (DYNLT1). It is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 21-343), a transmembrane domain (aa 344-367), and a cytoplasmic domain (aa 368-417). It contains two Ig-like C2 type domains (aa 145-237 and 233-328), and an Ig-like V-type domain that is shown to be necessary and sufficient for virus binding and uptake. CD155 also has one the immunoreceptor tyrosine-based inhibitor motif (ITIM; aa 396-401) that when phosphorylated by Src kinases binds to the SH2 domain of several SH2-containing phosphatases. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tahara-Hanaoka, S., et al. (2004). Int. Immunol. 16(4); 533-538; Iguchi-Manaka, A., et al. (2016). PLoS One. 11(4); e0152982). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |