Anti-Siglec-7/CD328 Antibody, clone 1B10 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1030 - recombinant, expressed in HEK 293 cells
Synonym: AIRM-1; CD328; CDw328; D-siglec; QA79 membrane protein; Sialic acid-binding Ig-like lectin 7; Siglec-7 Adhesion inhibitory receptor molecule 1; p75
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1030, Anti-Siglec-7/CD328, clone 1B10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong membranous/ECM staining was observed in both germinal center and non-germinal centers of human tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1030, Anti-Siglec-7/CD328, clone 1B10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong membranous/ECM staining was observed in both germinal center and non-germinal centers of human tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1030, Anti-Siglec-7/CD328, clone 1B10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong membranous/ECM staining was observed in both germinal center and non-germinal centers of human tonsil tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Raw264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1030, Anti-Siglec-7/CD328, clone 1B10 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Raw264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1030, Anti-Siglec-7/CD328, clone 1B10 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Raw264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1030, Anti-Siglec-7/CD328, clone 1B10 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from Jurkat (Lane 1), THP-1 (Lane 2), and HL-60 (Lane 3) cells were probed with Cat. No. ZRB1030, Anti-Siglec-7/CD328, clone 1B10 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Siglec-7/CD328 (~ 55 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 μg/mL of control peptide (Run 1) or Siglec-7/CD328 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1030, Anti-Siglec-7/CD328, clone 1B10 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Siglec-7/CD328 peptide displayed a KD value of 3.6 x 10-9 whereas its binding to control peptide displayed a KD of 1.1 x 10-3.
Flow Cytometry Enhanced Validation-Recombinant Antibody Technology Staining of one million Jurkat cells was performed using 1 μg of Cat. No. ZRB1030, Anti-Siglec-7/CD328, clone 1B10 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit IgG secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1B10, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 51.14 kDa |
| observed mol wt ~55 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9Y286  |
| Application: | Anti-Siglec-7/CD328, clone 1B10 ZooMAb, Cat. No. ZRB1030, is a Rabbit monoclonal antibody that detects Siglec-7/CD328 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Siglec-7/CD328 in Jurkat and HL-60 cell lysates. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Siglec-7/CD328 in Raw264.7 cells. Affinity Binding Assay: A representative lot of this antibody bound Siglec-7 peptide peptide with a KD of 3.6 x 10-9 in an affinity binding assay. Flow Cytometry Analysis: 1 μg from a representative lot detected Siglec-7/CD328 in one million Jurkat cells. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Siglec-7/CD328 in human tonsil tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal, cytoplasmic domain of human Sialic acid-binding Ig-like lectin 7 (Siglec-7/CD328). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1B10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Sialic acid-binding Ig-like lectin-7 (Siglec-7/CD328). It targets an epitope within 20 amino acids from the C-terminal, cytoplasmic domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Sialic acid-binding Ig-like lectin 7 (UniProt: Q9Y286; also known as Siglec-7 Adhesion inhibitory receptor molecule 1, AIRM-1, CDw328, D-siglec, QA79 membrane protein, p75, CD328) is encoded by the SIGLEC7 (also known as AIRM1) gene (Gene ID: 27036) in human. Siglec-7 is a single-pass type I membrane glycoprotein protein that is predominantly expressed by resting and activated natural killer (NK) cells. Its high expression is also observed in placenta, liver, lung, spleen, and peripheral blood leukocytes. Siglec-7 serves as an inhibitory receptor expressed on NK cells. Siglec-7 is also expressed on β-cells where it is shown to restore β-cell function and survival and reduce inflammation in pancreatic islets from patients with diabetes. Its levels are down-regulated in Type 1 and Type 2 diabetes. It preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid and also binds disialogangliosides. Siglec-7 is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that has an extracellular domain (aa 19-353), a transmembrane domain (aa 354-376), and a cytoplasmic domain (aa 377-467). Siglec-7 also contains one copy of immunoreceptor tyrosine-based inhibitor motif (ITIM) that is involved in modulation its cellular responses. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shao, JY., et al. (2016). Scand. J. Immunol. 84(3); 182-190; Dharmadhikari, G., et al. (2017). Sci. Rep. 7; 45319). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |