Anti-phospho-Stat3 (Tyr705) Antibody, clone 6C3, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1006 - recombinant, expressed in HEK 293 cells
Synonym: Acute-phase response factor; Signal transducer and activator of transcription 3
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:400 dilution of Cat. No. ZRB1006, Anti-phospho-Stat3 (Tyr705), clone 6C3 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear/membranous staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:400 dilution of Cat. No. ZRB1006, Anti-phospho-Stat3 (Tyr705), clone 6C3 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear/membranous staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:400 dilution of Cat. No. ZRB1006, Anti-phospho-Stat3 (Tyr705), clone 6C3 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear/membranous staining was observed in human breast cancer tissue sections.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Untreated cells (Lane 1) and SK-N-MC cells treated with Ciliary neurotrophic factor (Lane 2) were probed with Cat. No. ZRB1006, Anti-phospho-Stat3 (Tyr705), clone 6C3 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Stat3 (~88 kDa).
Peptide Inhibition Enhanced Validation-Recombinant Antibody Technology SK-N-MC cells treated with Ciliary neurotrophic factor were probed with Cat. No. ZRB1006, Anti-phospho-Stat3 (Tyr705), clone 6C3 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminesence detection system. Lane 1: Phospho Stat3 Tyr705 1:10,000 ran on SK-N-ME cells treated with Ciliary neurotrophic factor Lane 2: Phospho Stat3 Tyr705 1:10k tested with control peptide without phosphorylation on SK-N-ME cells treated with Ciliary neurotrophic factor Lane 3: Phospho Stat3 Tyr705 1:10,000 tested with peptide containing phosphorylation at Tyr705 on SK-N-ME cells treated with Ciliary neurotrophic factor Arrow indicates Stat3 ( ~88 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 6C3, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 88.07 kDa |
| observed mol wt ~88 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse, rat, monkey |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:100 |
| inhibition assay: 1:10,000 (peptide) | |
| western blot: suitable using 1:1,000 | |
| UniProt accession no. | P40763  |
| Application: | Anti-phospho-Stat3 (Tyr705), clone 6C3 ZooMAb, Cat. No. ZRB1006, is a recombinant Rabbit Monoclonal Antibody that specifically targets Stat3 phosphorylated on Tyrosine 705 and has been tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. |
| Application: | Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Stat3 in human breast cancer tissue sections. Peptide Inhibition Analysis: A 1:10,000 dilution from a representative lot was used with SK-N-MC cells treated with Ciliary neurotrophic factor for peptide block analysis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 11 amino acids from the C-terminal region of human Stat3 surrounding phospho tyrosine 705. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 6C3 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Stat3 phosphorylated on tyrosine 705. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Signal transducer and activator of transcription 3 (UniProt: P40763; also known as Stat3, Acute-phase response factor) is encoded by the STAT3 (also known as APRF) gene (Gene ID: 6774) in human. STAT3 is expressed in most cell types and is activated by multiple cytokines and growth factors. It is a latent cytoplasmic transcription factor that has a dual function of signal transduction and activation of transcription. It mediates cellular responses to interleukins and growth factors. STAT3 shuttles between the nucleus and the cytoplasm. STAT3 is activated by phosphorylation at Tyrosine 705, which induces dimerization, nuclear translocation and DNA binding. A motif comprising of amino acids 150-162 is essential for its nuclear import. In the nucleus it recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target genes. It binds to DNA as either a homodimer or as a heterodimer with STAT1. Constitutively activated STAT3 is reported in a number of human cancers. STAT3 regulates inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells. STAT3 may also play an apoptotic role by transactivating BIRC5 expression under LEP activation. Ad cytoplasmic STAT3 is shown to repress macroautophagy by inhibiting EIF2AK2/PKR activity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |