Anti-Histone H4 Antibody, clone 5J18, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1000 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and human colon (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10 (A) and 1:100 (B) dilution of Cat. No. ZRB1000, Anti-Histone H4, clone 5J18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Clone 5J18 exhibited the correct localization, but with low-intensity signal in human kidney (A) and human colon (B) tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and human colon (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10 (A) and 1:100 (B) dilution of Cat. No. ZRB1000, Anti-Histone H4, clone 5J18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Clone 5J18 exhibited the correct localization, but with low-intensity signal in human kidney (A) and human colon (B) tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and human colon (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10 (A) and 1:100 (B) dilution of Cat. No. ZRB1000, Anti-Histone H4, clone 5J18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Clone 5J18 exhibited the correct localization, but with low-intensity signal in human kidney (A) and human colon (B) tissue sections.
Western Blotting Enhanced Validation-Recombinant Antibody Technology BALB3T3/A31 acid extract was probed with Cat. No. ZRB1000, Anti-Histone H4, clone 5J18 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Histone H4 (~12 kDa).
Immunoprecipitation Enhanced Validation-Recombinant Antibody Technology 300 ng of Cat. No. ZRB1000, Anti-Histone H4, clone 5J18 ZooMAb® Rabbit Monoclonal was used to immunoprecipitate Histone H4 from 500 μg of BALB3T3/A31 acid extract. Immunoprecipitated 10 μg of BALB3T3/A31 acid extract (Lane 1), IP using control antibody (Lane 2), and IP using anti-Histone H4 antibody-clone 5J18 (Lane 3). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Histone H4 (~12 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 5J18, recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 11.37 kDa |
| observed mol wt ~12 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | Xenopus |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| western blot: suitable (peptide) | |
| UniProt accession no. | P62805  |
| Application: | Anti-Histone H4, clone 5J18 ZooMAb , Cat. No. ZRB1000, is a highly specific rabbit recombinant monoclonal antibody that targets Histone H4 and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Application: | Immunohistochemistry (Paraffin) Analysis: A 1:10-100 dilution from a representative lot detected Histone H4 in human kidney and human colon tissue sections. Immunoprecipitation Analysis: 300 ng from a representative lot immunoprecipitated Histone H4 in BALB3T3/A31 acid extract. Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H4. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 5J18 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects histone H4. It targets an epitope with in 10 amino acids from the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Histone H4 (UniProt P62805; also known as H4 histone family member A, Histone 1 H4a) is encoded by the HIST1H4A (also known as H4/A, H4FA) gene (Gene ID 8359) in human. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 is one of the main histone proteins that contribute to the chromatin structure in both prokaryotic and eukaryotic cells. Histone H4 can undergo several different types of epigenetic modifications that influence cellular processes, including methylation, acetylation, and phosphorylation. For example, Acetylation at Lys6, Lys9, Lys13 and Lys17 is shown to occur in coding regions of the genome but not in heterochromatin. Monomethylation and asymmetric dimethylation at Arg4 is reported to favor acetylation at Lys9 and Lys13. Histone H4 can be phosphorylated by Tie2 on Tyr51, which is shown to preferentially occur in the presence of core histones. The phosphorylated H4 is recognized by the proto-oncogene ABL1. Histone H4 is also phosphorylated on Ser48 by PAK2, which is shown to increase the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Chromosomal aberrations involving histone H4 causes of B-cell non-Hodgkin lymphomas. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |