Anti-GLUT-1 Antibody, CT, clone 16D21, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB071401 - recombinant, expressed in HEK 293 cells
Synonym: Glucose transporter type 1erythrocyte/brain; HepG2 glucose transporter; Solute carrier family 2 facilitated glucose transporter member 1
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and human placenta (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:250 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in trophoblasts of human placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and human placenta (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:250 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in trophoblasts of human placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and human placenta (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:250 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in trophoblasts of human placenta tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cells was performed using a 1:250 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cells was performed using a 1:250 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cells was performed using a 1:250 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal analysis of human placenta frozen sections was performed using a 1:100 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 dye (Green) This antibody positively stains the cytoplasm and plasma membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal analysis of human placenta frozen sections was performed using a 1:100 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 dye (Green) This antibody positively stains the cytoplasm and plasma membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal analysis of human placenta frozen sections was performed using a 1:100 dilution of Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 dye (Green) This antibody positively stains the cytoplasm and plasma membrane.
Western Blotting Enhanced Validation-Recombinant Antibody Technology HeLa cell lysate was probed with Cat. No. ZRB071401, Anti-GLUT-1, CT, clone 16D21 ZooMAb® Rabbit Monoclonal (1:250 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates GLUT-1 (~52 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 16D21, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 54.08 kDa |
| observed mol wt ~52 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | bovine, rat |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: 1:100-1:250 |
| immunofluorescence: suitable using 1:100 | |
| immunohistochemistry: 1:100-1:250 | |
| western blot: suitable using 1:1,000 | |
| UniProt accession no. | P11166  |
| Application: | Anti-GLUT-1, CT, clone 16D21 ZooMAb, Cat. No. ZRB071401, is arecombinant Rabbit Monoclonal Antibody that specifically targets GLUT1 and has been tested for use in Immunocytochemistry, Immunofluorescence., Immunohistochemistry, and Western Blotting, |
| Application: | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected GLUT-1 in human placenta tissue sections. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GLUT-1 in human placenta frozen sections. Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot detected GLUT-1 in NIH3T3 and HepG2 cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Solute carrier family 2, facilitated glucose transporter member 1 (GLUT-1). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 16D21 is a a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human and murine GLUT-1. It targets an epitope with in 12 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Solute carrier family 2, facilitated glucose transporter member 1 (UniProt: P11166; also known as Glucose transporter type 1, erythrocyte/brain, GLUT-1, HepG2 glucose transporter) is encoded by the SLC2A1 (also known as GLUT1) gene (Gene ID: 6513) in human. Glucose transporters are a family of integral membrane proteins that facilitative glucose uptake by cells. Seven different glucose transport proteins have been described that are designated as GLUT-1 to 7. GLUT-1 is a highly conserved; ubiquitously distributed, multi-pass membrane protein that is responsible for constitutive glucose uptake. It displays very broad substrate specificity and can transport a wide range of aldoses including both pentoses and hexoses. It is the predominant glucose transporter in embryonic and fetal tissues. In many organs, GLUT-1 is concentrated in endothelial cells of blood-tissue barriers. Hence, it has a specialized role to shuttle glucose between blood and organs that have limited access to small solutes via passive diffusion. It is also abundant in the mammalian erythrocyte membrane where it can rapidly equilibrate glucose between the cytoplasm of the erythrocyte and the blood plasma. GLUT-1 levels are reported to be frequently upregulated during tumorigenesis. Mutations in SLC2A1 gene are linked to GLUT-1 deficiency syndrome 1 and 2 that are characterized by infantile-onset epileptic encephalopathy, delayed development, microcephaly, and motor incoordination, and paroxysmal exercise-induced dyskinesia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |