Anti-Sirt1 (Sir2) Antibody, clone 1G16, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB07131 - recombinant, expressed in HEK 293 cells
Synonym: NAD-dependent protein deacetylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; SIR2alpha; Sir2; mSIR2a
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse testis (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:200 Dilution of Cat. No. ZRB07131, Anti-Sirt1 (Sir2), clone 1G16, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in seminiferous tubules of mouse testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse testis (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:200 Dilution of Cat. No. ZRB07131, Anti-Sirt1 (Sir2), clone 1G16, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in seminiferous tubules of mouse testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse testis (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:200 Dilution of Cat. No. ZRB07131, Anti-Sirt1 (Sir2), clone 1G16, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in seminiferous tubules of mouse testis tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH/3T3 cells was performed using a 1:100 dilution of Cat. No. ZRB07131, Anti-Sirt1 (Sir2), clone 1G16, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH/3T3 cells was performed using a 1:100 dilution of Cat. No. ZRB07131, Anti-Sirt1 (Sir2), clone 1G16, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of NIH/3T3 cells was performed using a 1:100 dilution of Cat. No. ZRB07131, Anti-Sirt1 (Sir2), clone 1G16, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation-Recombinant Antibody Technology BALB3T3/A31 Nuclear Extract was probed with Cat. No. ZRB07131, Anti-Sirt1 (Sir2), clone 1G16, ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Sirt1 (Sir2) (~110 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 1G16, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 80.32 kDa |
| observed mol wt ~110 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable using 1:100 |
| immunohistochemistry: 1:100-1:200 | |
| western blot: suitable using 1:1,000 | |
| UniProt accession no. | Q923E4  |
| Application: | Anti-Sirt1 (Sir2), clone 1G16, ZooMAb is a recombinant Rabbit Monoclonal antibody that detects NAD-dependent protein deacetylase sirtuin-1 (Sirt1) and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Application: | Immunohistochemistry (Paraffin) Analysis: A 1:200 dilution from a representative lot detected Sirt1 (Sir2) in mouse testis tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sirt1 (Sir2) in NIH/3T3 cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | His-tagged recombinant fragment corresponding to the first 131 amino acids from the N-terminal region of murine NAD-dependent protein deacetylase sirtuin-1 (Sirt-1). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1G16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects murine Sirt-1. It targets an epitope with in the CLOCK interacting region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | NAD-dependent protein deacetylase sirtuin-1 (UniProt: Q923E4; also known as Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2alpha, Sir2, mSIR2a, Sirt-1) is encoded by the Sirt1 (also known as Sir2l1) gene (Gene ID: 93759) in murine species. Sirt1 is a NAD-dependent protein deacetylase that is widely expressed and links transcriptional regulation directly to intracellular energetics. In liver, Sirt1 is expressed in a circadian manner with maximal and minimal levels reaching at around Zeitgeber time (ZT) 16 and ZT4, respectively and its deacetylase activity is also regulated in a circadian manner that peaks at ZT15. It participates in the coordination of several cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy. It is shown to modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. It deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression in both positive and negative manner. Sirt1 serves as a sensor of the cytosolic ratio of NAD+/NADH, which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Sirt1 has four nucleotide (NAD)-binding regions and four zinc binding sites. It is reported to shuttle between nucleus and cytoplasm. Sir1 is proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield a catalytic inactive but stable 75 kDa fragment (aa 2-525). Sirt1 can be phosphorylated by multiple protein kinases. It is phosphorylated by STK4/MST1 that results in inhibition of SIRT1-mediated p53/TP53 deacetylation and phosphorylation by MAPK8/JNK1 at serine 46 and threonine 522 is reported to increase its nuclear localization and enzymatic activity. Sirt1 phosphorylation at threonine 522 by DYRK1A and DYRK3 activates its deacetylase activity and can promote cell survival. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 2 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |