Anti-FADD Antibody, clone 11N7, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB06711 - recombinant, expressed in HEK 293 cells
Synonym: FAS-associated death domain protein; FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; Protein FADD
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A), Negative control (B) and Diluted 1:25 (C) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:500 dilution of Cat. No. ZRB06711, Anti-FADD, clone 11N7, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/ECM/Nuclear staining was observed in subsets of tubules of human kidney.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A), Negative control (B) and Diluted 1:25 (C) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:500 dilution of Cat. No. ZRB06711, Anti-FADD, clone 11N7, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/ECM/Nuclear staining was observed in subsets of tubules of human kidney.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A), Negative control (B) and Diluted 1:25 (C) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:500 dilution of Cat. No. ZRB06711, Anti-FADD, clone 11N7, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/ECM/Nuclear staining was observed in subsets of tubules of human kidney.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A), Negative control (B) and Diluted 1:25 (C) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:500 dilution of Cat. No. ZRB06711, Anti-FADD, clone 11N7, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/ECM/Nuclear staining was observed in subsets of tubules of human kidney.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from Jurkat cells (Lane 1) and MCF7 cells (Lane 2) were probed with Cat. No. ZRB06711, Anti-FADD, clone 11N7, ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates FADD (~26 kDa).
RNAi/siRNA/Gene Knockdown Enhanced Validation-Recombinant Antibody Technology A 1:250 dilution of Cat. No. ZRB06711, Anti-FADD, clone 11N7, ZooMAb® Rabbit Monoclonal was used on HeLa wild type (Lane 1) and FADD knockout (Lane 2) cell lysate. The image is a 10 min exposure using Immobilon Forte Western HRP substrate. The blocking was performed with Immobilon Signal Enhancer.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 11N7, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 23.28 kDa |
| observed mol wt ~26 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | gene knockdown: suitable using 1:250 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100 | |
| western blot: suitable using 1:1,000 | |
| UniProt accession no. | Q13158  |
| Application: | Anti-FADD, clone 11N7, ZooMAb, Cat. No. Cat. No. ZRB06711, is a recombinant Rabbit Monoclonal antibody that detects human FAS-associated death domain and has been tested for use in Immunohistochemistry (Paraffin), RNAi/siRNA/Gene Knockdown, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected FADD in MCF7 cell lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FADD in human kidney tissue sections. RNAi/siRNA/Gene Knockdown Analysis: A 1:250 dilution from a representative lot detected FADD lysates from Wild type HeLa cells, but not in FADD knockout HeLa cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | His-tagged full-length recombinant human FAS-associated death domain protein (FADD). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 11N7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human FAS-associated death domain protein (FADD). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | FAS-associated death domain protein (UniProt: Q13158; also known as FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD) is encoded by the FADD (also known as MORT1, GIG3) gene (Gene ID: 8772) in human. FADD is an apoptotic adaptor molecule that bridges death receptor signaling to the caspase cascade. It is expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. FADD contains a death domain at its C-terminus and a death effector domain (DED) at its N-terminal. The death domain is responsible for FADD binding to the activated trimerized Fas receptor. FADD recruits procaspase-8 through its N-terminal DED, which is then activated following the cleavage of pro domain. The death inducing signaling complex (DISC) formed by Fas receptor, FADD, and caspase-8 is essential in triggering apoptotic events in cells. Human FADD is phosphorylated mainly at Ser194. Defects in FADD gene have been linked to recurrent infections, hepatic dysfunction, and cardiovascular malformations. These subjects display high circulating levels of CD4(-) and CD8(-) cells and elevated levels of IL-10. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |