Anti-p44 MAPK (ERK1) Antibody, clone 13F10, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB06182 - recombinant, expressed in HEK 293 cells
Synonym: EC: 2.7.11.24; ERK-1; ERT2; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 3; MAP kinase isoform p44; MAPK 3; MNK1; Microtubule-
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat colon (A) and rat cerebral cortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB06182, Anti-p44 MAPK (ERK1), clone 13F10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous/nuclear staining was observed in glandular cells of rat colon and neurons of rat cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat colon (A) and rat cerebral cortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB06182, Anti-p44 MAPK (ERK1), clone 13F10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous/nuclear staining was observed in glandular cells of rat colon and neurons of rat cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat colon (A) and rat cerebral cortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB06182, Anti-p44 MAPK (ERK1), clone 13F10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous/nuclear staining was observed in glandular cells of rat colon and neurons of rat cerebral cortex tissue sections.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from Rat L6 (Lane 1), Rat C6 (Lane 2), and Mouse NIH/3T3 (Lane 3) cells were probed with Cat. No. ZRB06182, Anti-p44 MAPK (ERK1), clone 13F10 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates p44 MAPK (ERK1) (~44 and 42 kDa).
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysate from Human A431 cells was probed with Cat. No. ZRB06182, Anti-p44 MAPK (ERK1), clone 13F10 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates p44 MAPK (ERK1) (~44 and 42 kDa).
Immunoprecipitation Enhanced Validation-Recombinant Antibody Technology 100 μL of Cat. No. ZRB06182, Anti-p44 MAPK (ERK1), clone 13F10 ZooMAb® Rabbit Monoclonal was used to immunoprecipitate p44 MAPK (ERK1) in 500 μg of Rat L6 cell lysate. Immunoprecipitation reaction using L6 cell lysate and ZooMAb® Anti-p44-42 MAPK. Image is 30 sec exposure using forte. Lane 1 is 2% of input (10 μg of L6 lysate). Lane 2 is 15% of IP reaction (15 μL out of 100 run in lane) were then probed with Cat. No. ZRB06182, Anti-p44 MAPK (ERK1), clone 13F10 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using an Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates p44 MAPK (ERK1) (~44 and 42 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 13F10, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 43.08 kDa |
| observed mol wt ~44 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, rat, human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100 |
| immunoprecipitation (IP): suitable using 1:100 | |
| western blot: suitable using 1:1,000 | |
| UniProt accession no. | P21708  |
| Application: | Anti-p44 MAPK (ERK1), clone 13F10 ZooMAb, Cat. No. ZRB06182, is a recombinant Rabbit Monoclonal Antibody that detects p44 MAPK (ERK1) and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Application: | Western Blotting Analysis: 1:1,000 dilution from a representative lot detected p44 MAPK (ERK1) in Rat C6, L6, and mouse NIH/3T3 cell lysates. Immunoprecipitation Analysis: 100 μL from a representative lot immunoprecipitated p44 MAPK (ERK1) in Rat L6 cell lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected p44 MAPK (ERK1) in rat colon and rat cerebral cortex tissue sections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 35 amino acids from the C-terminal region of rat p44 MAPK (ERK1). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 13F10 is a ZooMAb rabbit recombinant monoclonal antibody that detects p44 MAPK (ERK1). May also display reactivity with p42 MAPK (ERK2). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Mitogen-activated protein kinase 3 (UniProt: P21708; also known as EC: 2.7.11.24, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1) is encoded by the Mapk3 (also known as Erk1, Prkm3) gene (Gene ID: 50689) in rat. ERK-1 is a serine/threonine kinase that acts as an essential component of the MAP kinase signal transduction pathway. It is expressed in different tissues, mostly in intestine, placenta, and lung. Highest levels have been reported in the nervous system. The MAPK/ERK cascade mediates several diverse biological processes such as cell growth, adhesion, survival, and differentiation through the regulation of transcription, translation, and cytoskeletal rearrangements. It also plays also a role in initiation and regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells by phosphorylating a few transcription factors. About 160 substrates have been described for ERKs. The protein kinase domain of ERK-1 is localized to amino acids 43-331 and the TXY motif is located in amino acids 203-205. ERK-1 is phosphorylated by MEK1 and MEK2 on threonine 203 and tyrosine 205 in response to external stimuli like insulin or nerve growth factor and both these phosphorylations are essential for its activity. Two isoforms of ERK-1 have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |