Anti-Tau-1 Antibody, clone PC1C6 ZooMAb® Mouse Monoclonal
SIGMA/ZMS3420 - recombinant, expressed in HEK 293 cells
Synonym: Microtubule-
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS3420, Anti-Tau-1, clone PC1C6 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in subsets of neurons, glia and neuropil of mouse cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS3420, Anti-Tau-1, clone PC1C6 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in subsets of neurons, glia and neuropil of mouse cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS3420, Anti-Tau-1, clone PC1C6 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in subsets of neurons, glia and neuropil of mouse cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS3420, Anti-Tau-1, clone PC1C6 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons and neuropil of rat cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS3420, Anti-Tau-1, clone PC1C6 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons and neuropil of rat cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS3420, Anti-Tau-1, clone PC1C6 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons and neuropil of rat cerebral cortex tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Rat E18 cortical cells was performed using a 1:100 dilution of Cat. No. ZMS3420, Anti-Tau-1, clone PC1C6 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the axons of neurons.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Rat E18 cortical cells was performed using a 1:100 dilution of Cat. No. ZMS3420, Anti-Tau-1, clone PC1C6 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the axons of neurons.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Rat E18 cortical cells was performed using a 1:100 dilution of Cat. No. ZMS3420, Anti-Tau-1, clone PC1C6 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the axons of neurons.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from SH-SY5Y cells (Left Lane) and Mouse brain tissue (Right Lane) were probed with Cat. No. ZMS3420, Anti-Tau-1, clone PC1C6 ZooMAb® Mouse Monoclonal (1:1,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG2a secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Tau-1 (~52-68 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse (recombinant) |
| clone | PC1C6, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG2a |
| mol wt | calculated mol wt 46.33 kDa |
| observed mol wt ~52-68 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human, rat |
| species reactivity (predicted by homology) | bovine |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P29172  |
| Application: | Anti-Tau-1, clone PC1C6 ZooMAb, Cat. No. ZMS3420, is a recombinant Mouse monoclonal antibody that specifically targets Tau-1 protein and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Tau-1 in mouse brain tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Tau-1 in mouse brain and rat brain tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Tau-1 in Rat E18 cortical cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | Purified denatured bovine microtubule associated proteins. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone PC1C6 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Microtubule-associated protein tau (Tau). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Microtubule-associated protein tau (UniProt: P29172; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (TAU) gene (Gene ID: 281296) in bovine species. Microtubule-associated protein tau is shown to be expressed in neurons. It is mostly found in axons, in the cytosol, and in association with plasma membrane components. Twenty isoforms of bovine Tau have been described that are generated by alternative splicing. Tau proteins promote microtubule assembly and stability and may also be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, thus serving as a linker protein between both. The short isoforms are reported to allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. In Alzheimer disease (AD), the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of Tau. In AD brain Tau accumulates in both the somatodendritic and axonal domains of neurons and it also accumulates in the soma as neurofibrillary tangles (NFTs). Tau oligomers are reported to be neurotoxic when applied extracellularly to cultured neuronal cells and can induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. It has been reported that most of the C-terminus of Tau in PHFs can be cleaved by trypsin, however the TauC4 region is highly resistant to trypsin action. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Taniguchi Watanabe, S., et al. (2016). Acta Neuropathol. 131(2); 267-280; Ando K., et al. (2010). Biochem. Soc. Trans. 38(4); 1001-1005). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |