Anti-HBV X-Protein Antibody, clone 227 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1083 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Western Blotting Enhanced Validation - Recombinant Antibody Technology Recombinant X-Protein of HBV-271.was probed with Cat. No. ZMS1083, Anti-HBV X-Protein, clone 227 ZooMAb® Mouse Monoclonal (1:20,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG1 secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates X-Protein (~18 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 10 μg/mL of non-specific GST protein (Run 1) or recombinant HBV X-Protein (Run 2) were analyzed by affinity binding assay using Cat. No. ZMS1083, Anti-HBV X-Protein, clone 227 ZooMAb® Mouse Monoclonal. The binding of this antibody to HBV X-Protein displayed a KD of 7.0 x 10-9 whereas binding to non-specific GST protein displayed a KD of 7.3 x 10-4.
ELISA Enhanced Validation - Recombinant Antibody Technology 50 ng of His-tagged full-length recombinant X-protein of HBV (Blue line) or GST control protein (Orange line) were analyzed by ELISA using various dilutions (starting at 3 μg/mL) of Cat. No. ZMS1083, Anti-HBV X-Protein, clone 227 ZooMAb® Mouse Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Mouse secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 227, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal half |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1κ |
| mol wt | calculated mol wt 16.58 kDa |
| observed mol wt ~18 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | Unknown  |
| purified by | using protein G |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | hepatitis B virus |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P69713 |
| Application: | Quality Control Testing Evaluated by Western Blotting with recombinant Protein X from HBV-271.] Western Blotting Analysis (WB): A 1:20,000 dilution of this antibody detected recombinant X-Protein of HBV-271. Tested Applications Affinity Binding Assay: A representative lot of this antibody bound HBV X-Protein with a KD of 7.0 x 10-9 in an affinity binding assay. Enzyme Immunoassay Analysis (ELISA): A representative lot of this antibody detected recombinant X-protein of Hepatitis B virus (HBV) in ELISA applications Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | A linear peptide corresponding to 26 amino acids from the C-terminal half of X Protein of Hepatitis B virus (HBV). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 227 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects X Protein of Hepatitis B virus. It targets an epitope within 26 amino acids from the C-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Protein X (UniProt: P69713; also known as HBx, Peptide X, pX) is encoded by the X gene (Gene ID: 944566) in Hepatitis B virus. Hepatitis B virus (HBV) contains seven main proteins, which include Core, pre-Core, Small S, Middle S, Large S, Polymerase, and the HBx protein. HBx, a multifunctional protein, although not essential for HBV infection, plays a role in silencing host antiviral defenses and promoting viral transcription. It facilitates the efficient replication of HBV by stimulating HBV gene expression from the cDNA template. It is mainly localized in the cytoplasm with a small fraction detected in the nucleus. However, its expression level can also influence its cellular localization. It is predominantly nuclear when expressed in cells at very low levels but becomes largely cytoplasmic as its expression level increases. In cytoplasm, a minor fraction may also associate with mitochondria. Its mitochondrial targeting sequence is localized in amino acids 68-117. HBx has an N-terminal negative regulatory domain and its transactivation or coactivation of the C-terminal domain is reported to interfere with host cell signal transduction transduction pathways to help HBV replication. The best-characterized HBx binding partner is the cellular damage-specific DNA binding protein 1 (DDB1) and the interaction of HBx-DDB1 is essential for HBV replication. HBx promotes the degradation of the cellular structural maintenance of chromosomes 5/6 complex (Smc5/6), which directly binds DNA and entraps DNA plasmids. HBx is directly involved in development of cirrhosis and liver and hepatocellular carcinoma. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kornyeyev, D., et al. (2019). J. Virol. 93(16); e00248-19; Hwang, G-Y., et al. (2003). J. Clin. Microbiol. 41(12); 5598-5603). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |