Monoclonal Anti-C20orf31 antibody produced in mouse
SIGMA/WH0055741M1 - clone 2E4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-C20orf49; Anti-EDEM2; Anti-FLJ10783; Anti-bA4204.1; Anti-chromosome 20 open reading frame 31
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to C20orf31 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 μg/mL]
Western Blotting Western Blot analysis of EDEM2 expression in transfected 293T cell line by C20orf31 monoclonal antibody, clone 2E4. Lanes Lane 1: EDEM2 transfected lysate (64.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of EDEM2 transfected lysate using anti-EDEM2 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with EDEM2 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged EDEM2 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_018217   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9BV94 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).[supplied by OMIM |
| Immunogen: | C20orf31 (NP_060687, 479 a.a. ~ 578 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PIDPAALHCCQRLKEEQWEVEDLMR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |