Monoclonal Anti-ATP6AP1 antibody produced in mouse
SIGMA/WH0000537M1 - clone 3A2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-16A; Anti-ATP6IP1; Anti-ATP6S1; Anti-ATPase, H+ transporting, lysosomal accessory protein 1; Anti-Ac45; Anti-CF2; Anti-MGC129781; Anti-ORF; Anti-VATPS1; Anti-XAP3
Product Type: Chemical
Western Blotting Western Blot analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody, clone 3A2. Lanes Lane 1: ATP6AP1 transfected lysate (52 kDa). Lane 2: Non-transfected lysate.
Western Blotting ATP6AP1 monoclonal antibody, clone 3A2. Western Blot analysis of ATP6AP1 expression in PC-12.
Western Blotting ATP6AP1 monoclonal antibody, clone 3A2 Western Blot analysis of ATP6AP1 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged ATP6AP1 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_001183   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15904 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. (provided by RefSeq) |
| Immunogen: | ATP6AP1 (NP_001174, 51 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SSDRDLWAPAADTHEGHITSDLQLS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |