Crystal Violet Solution
SIGMA/V5265 - 1%, aqueous solution
CAS Number: 548-62-9
Empirical Formula (Hill Notation): C25H30ClN3
Molecular Weight: 407.98
MDL Number: MFCD00011750
Linear Formula: C25H30ClN3
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: V5265-500ML.
| Amax | 0.36-0.44 at 589-594 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | deep violet, purple |
| concentration | 1% |
| form | aqueous solution |
| InChI | 1S/C25H30N3.ClH/c1-26(2)2 |
| InChI key | ZXJXZNDDNMQXFV-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | [Cl-].CN(C)c1ccc(cc1)C(c |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | • Crystal violet is commonly used in Gram staining for the classification of bacteria. • It has also been used to detect bacterial adherence to biomedical polymers and to stain DNA in mammalian tissues in Giemsa staining. • It has been successfully used to develop a counterion-staining method to detect DNA in agarose gel electrophoresis. • Its antimicrobial properties have facilitated its use in the treatment of oral candidiasis, skin infections, and methicillin-resistant Staphylococcus aureus. • It has been used as a stain in cell proliferation assays, migration assays, and Boyden chamber assay. |
| Application: | Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining. |
| Biochem/physiol Actions: | Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms entrapped complexes with Gram′s iodine that retain the purple color post-decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner. |
| Biochem/physiol Actions: | The antimicrobial effects of crystal violet can be attributed to free radical generation and formation of unionized bacterial complexes with the dye. It has also been reported to inhibit protein synthesis and bacterial cell wall formation. It is especially effective against Gram-positive bacteria. |
| General description: | Crystal violet, also known as gentian violet or methyl violet, is the N-hexamethylated derivative of pararosaniline. It is a basic, cationic, acidotropic protein dye used in histological and bacteriological staining. It is well-known for its antibacterial and antifungal properties. It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings. |
| Symbol |    GHS07,GHS08,GHS09 |
| Signal word | Warning |
| Hazard statements | H319 - H351 - H411 |
| Precautionary statements | P201 - P202 - P264 - P273 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | Xn,N |
| Risk Statements | 40-51/53 |
| Safety Statements | 36/37-61 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |