Urease from Canavalia ensiformis (Jack bean)
SIGMA/U7752
CAS Number: 9002-13-5
EC Number: 232-656-0
MDL Number: MFCD00070858
Product Type: Chemical
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| biological source | Canavalia ensiformis |
| contains | ~20% dithiothreitol |
| form | solid |
| mol wt | hexamer 545 kDa |
| trimer ~272 kDa | |
| Quality Level | 200  |
| storage temp. | −20°C |
| suitability | suitable for marker for electrophoresis (non-denaturing PAGE) |
| Application: | Urease from Canavalia ensiformis (Jack bean) has been used: • as a component of the molecular mass standard in electrophoresis • as a component of the standard in size-exclusion chromatography • as a reference in native-polyacrylamide gel electrophoresis (PAGE) |
| Biochem/physiol Actions: | Urease is an essential enzyme of the nitrogen cycle. It plays a role in degrading urea into ammonia and carbamate. Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense. |
| General description: | Subunit molecular weight: ~90,770 Composed of six subunits with total molecular weight: ~544,620 |
| General description: | Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria. It belongs to the superfamily of amidohydrolases and phosphotriesterases. Urease is also found in leguminous seeds. |
| Other Notes: | Contains approx. 1 mg protein |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.5.1.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |