Trypsin inhibitor from Glycine max (soybean)
SIGMA/T9767 - For use as a marker in SDS-PAGE, BioReagent
Synonym: SBTI
CAS Number: 9035-81-8
EC Number: 232-906-9
MDL Number: MFCD00082103
Product Type: Chemical
Trypsin Inhibitor (Kunitz) (soybean)
| biological source | Glycine max (soybean) |
| form | powder |
| mol wt | 20,000 Da |
| packaging | vial of 5 mg |
| product line | BioReagent |
| Quality Level | 200  |
| shipped in | ambient |
| solubility | balanced salt solution: 1 mg/mL |
| concentrate: >10 mg/mL, hazy, amber-yellow | |
| phosphate buffer: 10 mg/mL | |
| serum-free medium: soluble | |
| water: 10 mg/mL | |
| storage temp. | 2-8°C |
| technique(s) | electrophoresis: suitable |
| Application: | Trypsin inhibitor from Glycine max (soybean) has been used as a standard molecular weight marker in electrophoresis. |
| Biochem/physiol Actions: | This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent. |
| Other Notes: | One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginin |
| Other Notes: | The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges. |
| Preparation Note: | The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet. Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |