Tris-EDTA buffer solution

SIGMA/T9285 - 100X concentrate for Northern and Southern blotting

Synonym: Tris-EDTA wash buffer; Tris-EDTA wash buffer; TE buffer solution

MDL Number: MFCD00236359
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-T9285-100ML	100 mL	$31.50 1/EA		Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

application(s)	sample preparation
foreign activity	DNase, RNase, none detected
form	liquid
grade	Molecular Biology
InChI	1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key	VLEIUWBSEKKKFX-UHFFFAOYSA-N
pH	7.8-8.2 (1 × in water)
Quality Level	200
SMILES string	NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
sterility	0.2 μm filtered
storage temp.	room temp

Application:	TE buffer has been used for:• isolate, resuspend, or store DNA and RNA for PCR or qPCR • gel electrophoresis, including Northern and Southern blotting • bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells • antigen retrieval for immunohistochemistry
General description:	Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Preparation Note:	TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

RIDADR	NONH for all modes of transport
WGK Germany	nwg

Storage Temp.	room temp
UNSPSC	41105319

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