Anti-Acetylated Tubulin antibody, Mouse monoclonal
SIGMA/T7451 - clone 6-11B-1, purified from hybridoma cell culture
Synonym: Acetylated Tubulin Antibody; Acetylated Tubulin Antibody - Monoclonal Anti-Acetylated Tubulin antibody produced in mouse; Anti Acetyl Tubulin antibody; Anti Acetyl-alpha tubulin
MDL Number: MFCD00164512
Product Type: Chemical
Western Blotting JOURNAL CITATION: Class I and class II histone deacetylases are potential therapeutic targets for treating pancreatic cancer. By: Wang, G., He, J., et al. in PLoS One, 2012. PubMed ID: 23251689 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0052095), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: An essential role for DYF-11/MIP-T3 in assembling functional intraflagellar transport complexes. By: Li, C., Inglis, P. N., et al. in PLoS Genet, 2008. PubMed ID: 18369462 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1000044), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Class I and class II histone deacetylases are potential therapeutic targets for treating pancreatic cancer. By: Wang, G., He, J., et al. in PLoS One, 2012. PubMed ID: 23251689 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0052095), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Xenopus laevis labeled with Monoclonal Anti-Acetylated Tubulin (Cat. No. T7451). Staining shows the neuronal network in the tail. From Ellen Crabbe, DMBR, Kirsi Rilla, VIB-University Ghent, Zwijnaarde, Belgium.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 0.05 μg/mL Monoclonal Anti-Acetylated Tubulin Clone: 6-11B-1 (Cat. No. T7451). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 0.05 μg/mL Monoclonal Anti-Acetylated Tubulin Clone: 6-11B-1 (Cat. No. T7451). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.06 μg/mL Monoclonal Anti-Acetylated Tubulin Clone: 6-11B-1 (Cat. No. T7451). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. U87 3. COS7 4. P19 5. RAT2 6. CHO 7. MDBK 8. MDCK
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | 6-11B-1, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant |
| storage temp. | −20°C |
| target post-translational modification | acetylation (Lys40) |
| technique(s) | dot blot: suitable |
| electron microscopy: suitable | |
| immunohistochemistry: suitable | |
| radioimmunoassay: suitable | |
| western blot: 0.03-0.06 μg/mL using total rat brain extract | |
| UniProt accession no. | P68366  |
| Application: | Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in:• quantitative dot blot • immunofluorescence • Western blot • immunocytochemistry • enzyme-linked immunosorbent assay (ELISA) • solid-phase radioimmunoassay (RIA) • electron microscopy Anti-acetylated tubulin antibody has also been used in staining X-gal–stained paraffin sections to perform immunohistochemistry. |
| Biochem/physiol Actions: | Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin. |
| Biochem/physiol Actions: | The antibody has been used to detect acetylated α-tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corresponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila, and yeast. Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000. |
| General description: | Monoclonal anti-acetylated tubulin antibody (mouse IgG2b isotype) is derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 50,000 and share considerable homology. |
| Immunogen: | acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |